86 results found in Digital PCR
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faq
Can I combine multiple IFC runs in the Real-Time PCR Analysis software?
IFC runs with different assay sets can be combined as long as they have the same sample names. …
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faq
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faq
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User Guide
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Poster
ISSCR 2015: Correlating Single-Cell Transcript and Protein Expression During Reprogramming of Human Somatic Cells to Induced Pluripotent Stem (IPS) Cells
ISSCR 2015: Correlating Single-Cell Transcript and Protein Expression During Reprogramming of Human Somatic Cells to Induced Pluripotent Stem (IPS) Cells
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system
C1 Script Hub
Breakthrough single-cell applications, developed by and for the C1 community …
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general
Biomark & EP1 Support
Explore tutorials and publications, browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists. …
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system
EP1
Efficient, economical endpoint PCR results in hours with just minutes of hands-on time …
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Quick Reference
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article
Pioneering Progress in Cancer Immunotherapy
Single-cell genomics advances T cell immunotherapies at MD Anderson Cancer Center …
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faq
Can SYBR Green be used?
No, SYBR™ Green is not compatible with Fluidigm IFCs. …
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faq
What filters are included on the Biomark HD system?
The Biomark HD system comes with excitation sources and emission filters for FAM, VIC and ROX. …
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faq
Do you have any advice on primer design?
See these documents: …
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faq
Do I need preamplification for gene expression? Why?
Preamplification is recommended for all cDNA samples because of the nanoscale volume of Fluidigm IFCs. …
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faq
What is the maximum number of preamplification cycles I can do?
No maximum number of preamplification cycles has been established. …
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faq
Why is the Exo I treatment after preamplification needed only for EvaGreen?
EvaGreen® works by detecting all double-stranded DNA. …
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faq
What is the detection limit of target copy number per chamber for qPCR on the Biomark HD system? Is there a Ct cutoff?
The Biomark system can theoretically detect one copy per chamber, with a Ct value of 27−28 equating to single-copy. …
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faq
What is the basic method for generating hierarchical clusters?
Hierarchical clustering groups data in the form of a heat map that successively merges similar groups or data points. …
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faq
What is principal component analysis (PCA)?
PCA is a statistical method to reduce data containing a large number of variables into as few uncorrelated variables, or principal components, as possible. …
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faq
Why can’t I load the samples in the assay inlets and the assays in the sample inlets?
The sample and assay chambers inside the IFC are different sizes. …