218 results found in Digital PCR

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  Digital PCR

  Real-time results with 99.9% success and no compromises …

• Publications

  A certified plasmid reference material for the standardisation of BCR–ABL1 mRNA quantification by real-time quantitative PCR

  Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories. …

• Publications

  Tumor Content Chart-Assisted HER2/CEP17 Digital PCR Analysis of Gastric Cancer Biopsy Specimens

  Evaluating HER2 gene amplification is an essential component of therapeutic decision-making for advanced or metastatic gastric cancer. …

• Publications

  A Rapid Noninvasive Assay for the Detection of Renal Transplant Injury

  The copy number of donor-derived cell-free DNA (dd-cfDNA) in blood correlates with acute rejection (AR) in heart transplantation. …

• Publications

  Analysis of Extracellular RNA by Digital PCR

  The transfer of extracellular RNA is emerging as an important mechanism for inter-cellular communication. …

• Publications

  cep-1/p53-dependent dysplastic pathology of the aging C. elegans gonad

  The C. elegans germline and somatic gonad are actively developing until the animal reaches adulthood, and then continue to undergo striking changes as the animal ages. …

• Publications

  Changes in the regulation of the Notch signaling pathway are temporally correlated with regenerative failure in the mouse cochlea

  Sensorineural hearing loss is most commonly caused by the death of hair cells in the organ of Corti, and once lost, mammalian hair cells do not regenerate. …

• Publications

  Circulating Cell-Free Tumour DNA in the Management of Cancer

  With the development of new sensitive molecular techniques, circulating cell-free tumour DNA containing mutations can be identified in the plasma of cancer patients. …

• Publications

  Development of a Novel Reference Plasmid for Accurate Quantification of Genetically Modified Kefeng6 Rice DNA in Food and Feed Samples

  Reference plasmids are an essential tool for the quantification of genetically modified (GM) events. …

• Publications

  Current status and future potential of somatic mutation testing from circulating free DNA in patients with solid tumours

  Genetic alterations can determine the natural history of cancer and its treatment response. …

• Publications

  Catalytic mTOR inhibitors can overcome intrinsic and acquired resistance to allosteric mTOR inhibitors

  We tested the antitumor efficacy of mTOR catalytic site inhibitor MLN0128 in models with intrinsic or acquired rapamycin-resistance. …

• Publications

  Digital PCR for direct quantification of viruses without DNA extraction

  DNA extraction before amplification is considered an essential step for quantification of viral DNA using real-time PCR (qPCR). …

• Publications

  An Integrative Analysis of PIK3CA Mutation, PTEN, and INPP4B Expression in Terms of Trastuzumab Efficacy in HER2-Positive Breast Cancer

  The phosphoinositide-3-kinase (PI3K) pathway is commonly deregulated in breast cancer through several mechanisms, including PIK3CA mutation and loss of phosphatase and tensin homolog (PTEN) and inositol polyphosphate 4-phosphatase-II (INPP4B) …

• Publications

  Novel technologies and emerging biomarkers for personalized cancer immunotherapy

  The culmination of over a century’s work to understand the role of the immune system in tumor control has led to the recent advances in cancer immunotherapies that have resulted in durable clinical responses in patients with a variety of malignancies. …

• Publications

  Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

  DNA methylation is an important epigenetic mechanism in several human diseases, most notably cancer. …

• Publications

  Genetic and Functional Diversity of Propagating Cells in Glioblastoma

  Glioblastoma (GBM) is a lethal malignancy whose clinical intransigence has been linked to extensive intraclonal genetic and phenotypic diversity and the common emergence of therapeutic resistance. …

• Publications

  Granulocyte colony-stimulating factor prevents loss of spermatogenesis after sterilizing busulfan chemotherapy

  To determine whether granulocyte colony-stimulating factor (G-CSF) could prevent loss of spermatogenesis induced by busulfan chemotherapy via protection of undifferentiated spermatogonia, which might serve as an adjuvant approach to preserving male fertility among cancer patients. …

• Publications

  Assessment of the real-time PCR and different digital PCR platforms for DNA quantification

  Digital PCR (dPCR) is beginning to supersede real-time PCR (qPCR) for quantification of nucleic acids in many different applications. …

• Publications

  Nanofluidic Digital PCR for KRAS Mutation Detection and Quantification in Gastrointestinal Cancer

  Concomitant quantification of multiple mutant KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog) alleles may provide information in addition to that provided by standard mutation detection procedures. We assessed the feasibility of a nanofluidic digital PCR array platform to detect and quantify KRAS mutations simultaneously in clinically relevant samples. …

• Publications

  Digital PCR Analysis of Maternal Plasma for Noninvasive Detection of Sickle Cell Anemia

  Cell-free fetal DNA (cffDNA) constitutes approximately 10% of the cell-free DNA in maternal plasma and is a suitable source of fetal genetic material for noninvasive prenatal diagnosis (NIPD). The objective of this study was to determine the feasibility of using digital PCR for NIPD in pregnancies at risk of sickle cell anemia. …