79 results found in Digital PCR
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User Guide
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Quick Reference
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Quick Reference
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Quick Reference
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Quick Reference
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User Guide
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Protocol
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article
Pioneering Progress in Cancer Immunotherapy
Single-cell genomics advances T cell immunotherapies at MD Anderson Cancer Center …
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User Guide
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User Guide
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faq
Can SYBR Green be used?
No, SYBR™ Green is not compatible with Fluidigm IFCs. …
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faq
What filters are included on the Biomark HD system?
The Biomark HD system comes with excitation sources and emission filters for FAM, VIC and ROX. …
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faq
Do you have any advice on primer design?
See these documents: …
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faq
Do I need preamplification for gene expression? Why?
Preamplification is recommended for all cDNA samples because of the nanoscale volume of Fluidigm IFCs. …
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faq
What is the maximum number of preamplification cycles I can do?
No maximum number of preamplification cycles has been established. …
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faq
Why is the Exo I treatment after preamplification needed only for EvaGreen?
EvaGreen® works by detecting all double-stranded DNA. …
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faq
What is the detection limit of target copy number per chamber for qPCR on the Biomark HD system? Is there a Ct cutoff?
The Biomark system can theoretically detect one copy per chamber, with a Ct value of 27−28 equating to single-copy. …
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faq
What is the basic method for generating hierarchical clusters?
Hierarchical clustering groups data in the form of a heat map that successively merges similar groups or data points. …
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faq
What is principal component analysis (PCA)?
PCA is a statistical method to reduce data containing a large number of variables into as few uncorrelated variables, or principal components, as possible. …
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faq
Why can’t I load the samples in the assay inlets and the assays in the sample inlets?
The sample and assay chambers inside the IFC are different sizes. …