660 results found for "PROTOCOL"
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faq
Have you tested these antibodies using automated staining protocols?
Fluidigm has not tested the antibodies using automated staining protocols, but some of our customers are experimenting with these. …
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faq
Where can I find standard CyTOF® system mass cytometer protocols?
Our protocols can be found on our website. …
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faq
How hard is it to label an antibody?
Maxpar® kits use a simple chemistry, and the process takes a few hours. …
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faq
How do I estimate read depth coverage?
Guidelines for determining the estimated read depth are provided within the Advanta™ CFTR NGS Library Prep Assay protocol. …
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Customer Bulletin
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faq
How is this new cell staining protocol different from previous Fluidigm protocols, and what makes it better?
The biggest change is that the updated protocols all call for use of freshly prepared 1.6% formaldehyde solution. Fluidigm recommends using ampules of Pierce™ 16% (w/v) Formaldehyde, Methanol-free (Thermo Scientific™ Cat. No. 28906, 10 x 1 mL). Other sources of fixative are available. Please contact your field applications scientist for more information. Optimal staining and cell integrity is only obtained using fixative prepared from a new ampule daily. …
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faq
Can I change the thermal cycling conditions for my genotyping reactions?
While it is possible to modify existing thermal protocols or create custom thermal protocols, Fluidigm does not support protocol variations other than those listed in the SNP Genotyping User Guide (PN 68000098), and any deviations are at the risk of the user. …
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faq
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Customer Bulletin
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Protocol
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faq
How many protocols can Flow Conductor run at the same time?
Flow Conductor™ features 28 positions for secondary tubes. Up to 28 protocols can be executed in a single run. This means each tube can have its own protocol. …
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faq
Should I implement the updated cell staining protocols immediately?
If you are starting any new studies or sets of experiments, we recommend that you use the updated staining protocols. If new experiments are to be part of an ongoing study, we strongly suggest that you perform a bridging study first. Contact your Fluidigm field applications scientist if you are unsure how to proceed with such studies. …
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faq
Does Flow Conductor provide time savings for flow or mass cytometry protocols?
Flow Conductor™ delivers significant hands-on time savings compared to manual workflows. Just load specimens, reagents and target tubes, and then start your protocol for fully automated sample processing. …
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faq
What are the new protocols for mass cytometry that I have been hearing about?
Fluidigm has released updated protocols for all cell staining and fixation procedures. The most important update is the use of freshly prepared formaldehyde fixative each day that cells are stained. The updated protocols can be found here. …
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Protocol
C1 System for microRNA (TaqMan) Protocol
Using C1 to Capture Cells from Cell Culture and Perform Preamplification of MicroRNA Species Using TaqMan Assays
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Protocol
C1 System for mRNA Seq Protocol
Using C1 to Generate Single-Cell cDNA Libraries for mRNA Sequencing