1415 results found for "SNP Type assays"
D3 Assay Design
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faq
What are SNP Type Assays?
SNP Type Assays are allele-specific PCR assays made by Fluidigm that use three primers and two universal probes to distinguish between two alleles. …
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faq
How should I store my SNP Type Assays?
SNP Type Assays are stable for up to six months at −20 °C if 96-well plates remain sealed to avoid evaporation. …
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faq
Why can't I run real-time PCR with my SNP Type Assays?
SNP Type Genotyping reactions must be imaged at room temperature (25 °C) so that any unincorporated universal probes will be hybridized to their respective quencher oligos. …
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faq
Can SNP Type assays be wet-lab tested?
No, SNP Type assays cannot be ordered wet lab-tested. …
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faq
How do I design and order SNP Type assays?
SNP Type assays can be designed and ordered through the D3 assay design on the Fluidigm website. …
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faq
Can SNP Type assays be designed to targets with adjacent SNPs?
Target SNPs that contain adjacent SNPs within 20 base pairs on either side will not be designed. …
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faq
What is provided with my order of SNP Type assays?
SNP Type assays are provided in three separate oligo plates per 96 assays. …
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data sheet
SNP Type Assays
Fluidigm SNP Type Assays provide a high-throughput, low-cost single nucleotide polymorphism (SNP) genotyping solution which enables rapid assay design and polymorphism screening.
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faq
Does Fluidigm design SNP Type assays for nonhuman species?
Yes. Please specify species type and provide sequences when using the D3 assay design service. …
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faq
What are the SNP Type Assay performance specifications?
See the SNP Type Assays Data Sheet (PN 100-3464). …
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faq
How do the SNP Type and TaqMan chemistries differ?
Both chemistries are robust methods for SNP genotyping, and both are compatible with specific target amplification protocols. …
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faq
What fluorophores are used for SNP Type Genotyping? How do I know which fluorophore represents which allele?
SNP Type Genotyping Assay probes are labeled with the FAM and HEX fluorophores. …
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faq
What genotyping chemistries can be used on the Fluidigm platform?
Fluidigm supports the use of its SNP Type assays and the TaqMan® SNP Genotyping Assays from Thermo Fisher Scientific. …
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faq
What types of SNPs can be targeted with D3 assay design?
We currently offer designs for bi-allelic SNPs only. …
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faq
Can I use any of the Fluidigm Sample Loading Reagents for my genotyping sample preparation?
No. Each of the available genotyping protocols has been optimized for best performance, and only the specified Sample Loading Reagent should be used. …
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product insert
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faq
How many bases upstream and downstream of target SNPs do I need to provide when submitting a D3 assay design request?
A minimum of 60 base pairs (bp) upstream and 60 bp downstream of the targeted SNP is recommended. …
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faq
What PCR master mixes are supported for genotyping?
Fluidigm currently supports the use of the following master mixes: …
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faq
What is the minimum genomic DNA (gDNA) concentration required for genotyping on the Biomark, Biomark HD or EP1 system? Why?
Fluidigm genotyping technology partitions each sample into multiple reaction chambers on the IFC. …
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faq
What is the turnaround time for D3 assay design?
D3 assay design makes designs available for review within 72 hours of submission. …