188 results found for "digital PCR" in Digital PCR
Digital PCR
Digital PCR (dPCR) on the Biomark™ system delivers unparalleled accuracy and real-time dPCR capabilities that virtually eliminate false positives. The platform provides a choice of real-time or endpoint analysis and the capability to quantitate up to four targets per sample using multiple reporter dyes. Innovative software enables automated copy number variation measurement as well as quantitation. Our digital PCR technology provides efficient, economical results. And did we mention the 99.9% success rate?
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User Guide
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consumable
Digital PCR
Real-time results with 99.9% success and no compromises …
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Publications
Viral diagnostics in the era of digital PCR
Unlike quantitative PCR (qPCR), digital PCR (dPCR) achieves sensitive and accurate absolute quantitation of a DNA sample without the need for a standard curve. …
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Publications
Analysis of Extracellular RNA by Digital PCR
The transfer of extracellular RNA is emerging as an important mechanism for inter-cellular communication. …
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consumable
12.765 Digital Array™ IFC for Digital PCR
One 12.765 Digital Array IFC, which enables 9,180 reactions using 12 samples. Control Line Fluid syringes and reagents sold separately. …
Product #: BMK-M-12.765
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consumable
48.770 Digital Array™ IFC for Digital PCR
One 48.770 Digital Array IFC, which enables 36,960 reactions using 48 samples. Control Line Fluid syringes and reagents sold separately. …
Product #: BMK-M-48.770
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Publications
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. …
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faq
Can I run EvaGreen chemistry on the Digital PCR IFCs?
Yes, with the release of the qdPCR 37K™ IFCs, Fluidigm implemented a software upgrade that includes an option to run EvaGreen (v4.0.1 and later). …
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Publications
Methods for Multiplex Template Sampling in Digital PCR Assays
The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. …
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Publications
Assessment of the real-time PCR and different digital PCR platforms for DNA quantification
Digital PCR (dPCR) is beginning to supersede real-time PCR (qPCR) for quantification of nucleic acids in many different applications. …
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Publications
Molecular quantification of environmental DNA using microfluidics and digital PCR
Real-time PCR has been widely used to evaluate gene abundance in natural microbial habitats. …
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Publications
Accurate quantification of supercoiled DNA by digital PCR
Digital PCR (dPCR) as an enumeration-based quantification method is capable of quantifying the DNA copy number without the help of standards. …
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faq
How is the concentration per panel calculated in Digital PCR?
The concentration per panel can be calculated based on: …
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faq
What is the recommended input concentration for Digital PCR IFCs?
A range of concentrations can be used. …
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Publications
The applicability of digital PCR for the assessment of detection limits in GMO analysis
We investigate the applicability of using digital PCR to estimate absolute limits of detection and quantitation associated with the validation of traditional real-time PCR methods for analysis of genetically modified (GM) ingredients. …
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Publications
Nanofluidic Digital PCR for KRAS Mutation Detection and Quantification in Gastrointestinal Cancer
Concomitant quantification of multiple mutant KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog) alleles may provide information in addition to that provided by standard mutation detection procedures. We assessed the feasibility of a nanofluidic digital PCR array platform to detect and quantify KRAS mutations simultaneously in clinically relevant samples. …
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Publications
Microfluidic digital PCR enables rapid prenatal diagnosis of fetal aneuploidy
The purpose of this study was to demonstrate that digital polymerase chain reaction (PCR) enables rapid, allele independent molecular detection of fetal aneuploidy. …
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Publications
Evaluation of Digital PCR for Absolute RNA Quantification
Gene expression measurements detailing mRNA quantities are widely employed in molecular biology and are increasingly important in diagnostic fields. …
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