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54 results found for "permeabilization"

  • faq

    Can I stain both cell surface and intracellular targets simultaneously when preparing cells for mass cytometry?

    Yes. The standard protocols for staining intracellular targets involve fixing followed by permeabilization of the cells, which allows probe access to both cell surface and intracellular targets. …

  • consumable

    Maxpar® Fix I Buffer—50 mL

    Optimized for fixation of cells prior to permeabilization with the Maxpar Permeabilization Buffer when performing intracellular staining of antigens located in the cytoplasm and secretory pathway. …

    Product #: 201065

  • Protocol

  • product insert

  • consumable

    Cell-ID™ Cisplatin-195Pt—100 µL

    Cell-ID™ Cisplatin-195Pt is a monoisotopic preparation of cisplatin-containing the 195Pt isotope. Cisplatin is a stain that binds covalently to cellular proteins and labels cells with compromised cell membranes to a greater extent than live cells. Cell-ID Cisplatin-195Pt therefore specifically identifies dead cells if cells are incubated prior to fixation, or it identifies total cells if cells are incubated following cell fixation and permeabilization. Because cisplatin binds covalently to protein, its labeling remains strong through subsequent cell handling steps used in downstream mass cytometry cell staining protocols. …

    Product #: 201195

  • consumable

    Cell-ID™ Cisplatin-196Pt—100 µL

    Cell-ID™ Cisplatin-196Pt is a monoisotopic preparation of cisplatin-containing the 196Pt isotope. Cisplatin is a stain that binds covalently to cellular proteins and labels cells with compromised cell membranes to a greater extent than live cells. Cell-ID Cisplatin-196Pt therefore specifically identifies dead cells if cells are incubated prior to fixation, or it identifies total cells if cells are incubated following cell fixation and permeabilization. Because cisplatin binds covalently to protein, its labeling remains strong through subsequent cell handling steps used in downstream mass cytometry cell staining protocols. …

    Product #: 201196

  • tds

  • product insert

  • consumable

    Cell-ID™ Cisplatin—100 µL

    Cell-ID Cisplatin specifically identifies dead cells when incubated prior to fixation or identifies total cells when incubated following cell fixation and permeabilization. Containing a mixture of naturally occurring platinum isotopes, it is best detected in the 195 channel. …

    Product #: 201064

  • consumable

    Maxpar® Perm-S Buffer—250 mL

    Optimized for permeabilization of cells following cell fixation with the Maxpar Fix I Buffer when performing intracellular staining of antigens located in the cytoplasm and secretory pathway. …

    Product #: 201066

  • consumable

    Maxpar® Fix and Perm Buffer—100 mL

    Optimized for a single-step cell fixation, permeabilization, and intercalation. It also facilitates short-term storage of CyTOF® stained samples prior to analysis. …

    Product #: 201067

  • consumable

    Cell-ID™ Cisplatin-198Pt—100 µL

    Cell-ID™ Cisplatin-198Pt is a monoisotopic preparation of cisplatin-containing the 198Pt isotope. Cisplatin is a stain that binds covalently to cellular proteins, and labels cells with compromised cell membranes to a greater extent than live cells. Cell-ID Cisplatin-198Pt therefore specifically identifies dead cells if cells are incubated prior to fixation, or it identifies total cells if cells are incubated following cell fixation and permeabilization. Because cisplatin binds covalently to protein, its labeling remains strong through subsequent cell handling steps used in downstream mass cytometry cell staining protocols. …

    Product #: 201198

  • consumable

    Cell-ID™ Cisplatin-194Pt—100 µL

    Cell-ID™ Cisplatin-194Pt is a monoisotopic preparation of cisplatin-containing the 194Pt isotope. Cisplatin is a stain that binds covalently to cellular proteins and labels cells with compromised cell membranes to a greater extent than live cells. Cell-ID Cisplatin-194Pt therefore specifically identifies dead cells if cells are incubated prior to fixation, or it identifies total cells if cells are incubated following cell fixation and permeabilization. Because cisplatin binds covalently to protein, its labeling remains strong through subsequent cell handling steps used in downstream mass cytometry cell staining protocols. …

    Product #: 201194

  • consumable

    Maxpar® 10X Barcode Perm Buffer—50 mL

    Maxpar® Barcode Perm Buffer is optimized for the permeabilization of cells following fixation with Maxpar Fix I Buffer when barcoding samples. …

    Product #: 201057

  • consumable

    Anti-Human VEGF (G153-694)-163Dy—25 µg

    VEGF is a highly specific mitogen for vascular endothelial cells and induces angiogenesis and permeabilization of blood vessels. It is secreted by pituitary cells, monocytes/macrophages, smooth muscle cells and keratinocytes. …

    Product #: 3163028D

  • Poster

    CYTO 2021: Compatibility of new platinum-conjugated antibodies with standard CyTOF workflows 

    Huihui Yao, Michael Cohen, Carlene Petes, Daniel Majonis, Christina Loh

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