High-Plex. High-Throughput.

Sample multiplexing by cell barcoding for CyTOF.

Sample-specific cell barcoding for simplified workflows and improved staining consistency

Cell barcoding tags each sample with a unique identifier, enabling sample multiplexing for improved workflows with CyTOF® systems and enhanced data quality.

With cell barcoding, achieve higher throughput for scaled-up experiments and even greater data consistency for large studies.

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Combine more samples at once to run larger experiments with 35-plus samples.

Integrate barcodes with CyTOF panels without compromising panel size.

Improve data quality and streamline workflows.

How barcoding works

How barcoding works
  1. Individual samples are barcoded with a unique metal isotope code.
  2. Barcoded samples are combined into a single, multiplexed sample.
  3. The multiplexed sample is stained in a single tube.
  4. The multiplexed sample is acquired on a Helios™ instrument
  5. Data are debarcoded for individual sample analysis.

Benefits of barcoding

Accessibility Accessibility: Uses isotopes and workflows that are compatible with existing panel designs

Scaled-up experiments Scaled-up experiments: Reduces processing time in experimental workflow, making it easier to increase sample throughput

Sample conservation Sample conservation: Enables efficient use of precious samples by allowing you to combine many samples in one tube
Increased consistency Increased consistency: Eliminates sample-to-sample variation introduced when samples are stained and collected individually

Cleaner data Cleaner data: Filters inter-sample doublets and multiplets by detecting more than one barcode per event

Reagent savings Reagent savings: Lower, more economical use of antibodies and reagents

Barcoding methods

  Palladium-Based Barcoding   Live-Cell Barcoding
Product Cell-ID™ 20-Plex Pd Barcoding Kit (PN 201060) Maxpar® anti-human CD45 antibodies
Types of cells Universal, cell-agnostic Best for human leukocytes, CD45-negative cells excluded
Configuration Ready-to-use kit and accompanying software Customer-designed barcoding scheme and individual anti-CD45 antibody selection
Fix/perm requirements Fix/perm steps in workflow, usable with intracellular staining protocols including phosphostaining No fix/perm required prior to staining with panel
Panel integration Adjustments to existing panels or workflows may be needed due to fix/perm step. Easy to layer onto existing panels
Number of samples Barcode up to 20 samples. Flexible design allows scaling for sample number to 50-plus
Resources Application Note: The Benefits of Palladium Barcoding on Data Quality and Workflow Technical Note: Using Monoisotopic Platinum-Containing Reagents for Suspension Mass Cytometry

Application Note: Enabling Live-Cell Barcoding with Anti-CD45 Antibodies in Suspension Mass Cytometry


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