The Cell-ID™ 20-Plex Pd Barcoding Kit enables barcoding of up to 20 samples, which are then stained and acquired as a single multiplexed sample. The kit contains all the necessary reagents, and the protocol can be combined with all previous CyTOF® workflows.
Workflow
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Barcode
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Combine
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Stain
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Acquire
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Debarcode
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Analyze
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Contents
| Component | Volume |
| 3 sets of 20 barcodes in PCR tubes | 10 µL each tube |
| Maxpar® 5X Fix I Buffer | 15 mL |
| Maxpar Cell Staining Buffer | 500 mL |
| Maxpar 10X Barcode Perm Buffer | 50 mL |
| Maxpar PBS | 500 mL |
Technical Support
See our product spec sheet and user guide for technical details. For additional technical support, visit fluidigm.com/support to download user guides, protocols and more.
References
Read more about barcoding for CyTOF systems in these publications.
Behbehani, G.K., Thom, C., Zunder, E.R. et al. "Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining." Cytometry 85 (2014): 1011–1019.
Zunder, E.R., Finck, R., Behbehani, G.K. et al. "Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm." Nature Protocols 10 (2015): 316–333.