Imaging Mass Cytometry
Imaging Mass Cytometry™ (IMC™) is the most trusted technology that enables researchers to accurately assess complex phenotypes and immune spatial interactions in the tissue microenvironment.
Explore mechanism of action, disease progression and therapeutic outcomes – with confidence.
Keep current with how IMC is used to gain deep spatial insights – see articles, news, publications, bibliographies, tips and more on our IMC Trending Topics page.
Discover the throughput and precision that is uniquely designed for translational researchers.
Imaging Mass Cytometry generates high-dimensional spatial data at subcellular resolution. IMC uses cytometry by time-of-flight (on which CyTOF® systems are based) to overcome the multiplexing limitations of traditional immunohistochemistry (IHC) and immunofluorescence. By applying metal-tagged antibodies instead of fluorochromes, IMC has the unique capability to simultaneously stain, acquire and analyze 40-plus markers of interest on a tissue section without interference from autofluorescent tissues or management of spectral overlap.
IMC is the only technology with
No autofluorescence interference to image any tissue type
40-plus markers imaged simultaneously to get results faster
Protein and RNA co-detection for deeper insights
Integrated cell segmentation for faster interpretation
Batch staining of all slides for high-volume studies
Dual imaging and flow cytometry mode to maximize investment
IMC shows the true biology
High-plex imaging for all tissue types ― including lung, bone marrow, colon and brain ― without autofluorescence interference.
- Well-defined red signals from CD68
- Cellular structure is sharply defined by yellow pan-cytokeratin stain
- CD68 indistinct or missing
- Cellular structure diffuse
Reasons to choose IMC for your high-plex imaging study
WORKFLOW
Get results faster
Hyperion™ Imaging Systems use a one-step staining and detection approach that enables samples to be simultaneously stained, acquired and analyzed.
1
Modularized panels
Swap markers without panel revalidation.
2
Simultaneous staining
Stain 40-plus markers for all slides at once.
3
One-step detection
Simultaneous imaging of 40-plus markers, including protein and RNA.
4
Precise signals
Image any tissue without autofluorescence.
5
Real-time analysis
Visualize 40-plus markers in 30 minutes.
A one-step staining and detection workflow
Imaging Mass Cytometry enables 40-plus markers that can be simultaneously stained, acquired and visualized. Other fluorescence-based approaches involve iterative rounds of staining, imaging and removal of fluorescent signals. The IMC workflow is without sequential immunostaining approaches, multiple slide treatment rounds or acquisition steps.
Need to ship or store slides?
- All-at-once batch staining of all slides to reduce technical variation
- Acquire at any time from shipped and/or stored slides that have been stained
- Analyze previously banked tissue slides to be correlated to known clinical outcomes
A workflow ideal for high-volume samples,
clinical research trials and multi-site studies
Applications
Mar 26 - Mar 28
Location
FlowTex (Texas Flow Cytometry User Group) in Houston,TX
Apr 05 - Apr 10
Location
AACR (American Association for Cancer Research) in San Diego, CA
Apr 08 - Apr 09
Location
SoCal Flow (Southern California Flow Cytometry Association) in Irvine, CA
Apr 09
Location
Midlands Innovations Flow Cytometry Meeting in Leicester, UK
Apr 09 - Apr 11
Location
BSAS Conference (British Society of Animal Science) in Belfast, UK
Apr 09 - Apr 12
Location
Analytica 2024 in München, Germany
Apr 15
Location
X-omics Festival 2024 in Nijmegen, the Netherlands
Apr 21 - Apr 24
Location
ABRF (Association of Biomolecular Resources Facilities) in Minneapolis, MN
Apr 25 - Apr 26
Location
Immuno 2024 in London, UK
Apr 25
Location
GW4 Cytomics Symposium in Exeter, UK
Resources
Flyers and brochures
Spotlight articles
Application notes
Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.