Maxpar Antibody Conjugation Service for Mass Cytometry

Expand your CyTOF panels with custom isotope-conjugated antibodies

Highlights

Flexible—Extend your mass cytometry panels by adding metal-labeled targets not yet available in the Maxpar catalog.

Efficient—Save time and resources by letting the experts do the labeling and quality control for you.

Dependable—Conjugation service performed by our professional technicians ensures product with consistently high quality and reliable performance.

Mass cytometry is the most comprehensive way to profile cell phenotype and function, enabling simultaneous detection of more than 40 cellular targets in every precious sample.

Whether you’re building a 15- or 40-parameter mass cytometry panel, the extensive Fluidigm catalog of preconjugated antibodies and antibody panel kits will get you there.

We understand your panel design needs. If you have a proprietary antibody to add to your panel or require customized design, our Maxpar® Antibody Conjugation Service is committed to your success.

Select the desired isotope from our 37 high-purity metal tags, decide which confirmation method you prefer—biochemical analysis or more extensive biologic functional testing—and place your order.

After our expert scientists perform the conjugation and analysis, we’ll send your antibody conjugate along with a detailed analytic report.

Custom Conjugation Workflow


Conjugation Workflow

Custom Conjugation Service Specifications and Ordering Information

Antibody Conjugation Service with Biological Confirmation

Antibody Conjugation Service with Biochemical Confirmation

Biochemical analysis provided Yes Yes
Biological confirmation performed Yes
(Available for Helios™/CyTOF® only)*
No
Staining profile and titer data Yes Yes
Timeline 6 to 8 weeks from receipt of antibody 4 to 6 weeks from receipt of antibody

Yield

Standard scale Input 200 µg
Purified, protein-free buffer
Cat. No. 3999999-1
Yield: 100 tests (1–3 x 10
6 cells)
Cat. No. 3999999-2
Yield: 40 µg minimum
Bulk scale
Variable input mass of antibody
Purified, protein-free buffer
Cat. No. 3999999-5
Extra 100-test vial Additional units of 100 tests each
Cat. No. 3999999-7
Yield: 200 µg minimum
Cat. No. 3999999-8
Yield: 600 µg minimum
*Not available for antibodies used on the Hyperion™ Imaging System

Ligands and buffer suitable for custom conjugation—IgG monoclonal and polyclonal antibodies are routinely conjugated. The feasibility of conjugating other antibody isotypes and proteins is considered on a case-by-case basis. To facilitate labeling, ligands must be provided purified, unconjugated and in a protein-free buffer at minimum concentration of 0.5 mg/mL.

Source of antibodies—Antibodies to be custom-conjugated may be either provided by the customer or purchased from a commercial source. Where Fluidigm purchases an antibody on behalf of a customer, the cost is quoted and billed as a separate line item, Cat. No. 3999999-3, Antibody Material Handling Charge.

Quality control—All custom conjugations are subject to rigorous biochemical confirmation, including inductively coupled plasma mass spectrometry, determination of concentration and yield and assessment of ratio of attachment of metal atoms to antibody. Optionally available biological confirmation service involves empirical testing by CyTOF on a cell line, with optimal titer determined and the conjugate formulated for delivery in a 100-test vial.

Contact Us to learn more about Maxpar Custom Antibody Conjugation Services.

Download the Maxpar Custom Antibody Conjugation Services flyer.


For Research Use Only. Not for use in diagnostic procedures.