Easily combine up to 20 samples with ready-to-use universal cell barcoding kit.
The Cell-ID™ 20-Plex Pd Barcoding Kit (PN 201060) uses a combination of three of six stable palladium (Pd) isotopes to identify up to 20 fixed and permeabilized samples of any cell type, independent of species.
The kit combines the necessary reagents and barcoding tags to let you integrate barcoding into your next experiment.
This method is compatible with downstream staining of surface, intracellular, nuclear and phosphorylated antigen targets that are not affected by fixation.
The Benefits of Palladium Barcoding on Data Quality and Workflow
Benefits of barcoding
|Accessibility: Uses isotopes and workflows that are compatible with existing panel designs
|Scaled-up experiments: Reduces processing time in experimental workflow, making it easier to increase sample throughput
|Sample conservation: Enables efficient use of precious samples by allowing you to combine many samples in one tube|
|Increased consistency: Eliminates sample-to-sample variation introduced when samples are stained and collected individually
|Cleaner data: Filters inter-sample cell doublets and multiplets by detecting more than one barcode per cell
|Reagent savings: Lower, more economical use of antibodies and reagents|
|Palladium-based barcoding method|
|Product||Cell-ID™ 20-Plex Pd Barcoding Kit (PN 201060)|
|Types of cells||Universal, cell-agnostic|
|Configuration||Ready-to-use kit and accompanying software|
|Fix/perm requirements||Fix/perm steps in workflow, usable with intracellular staining protocols including phosphostaining|
|Panel integration||Adjustments to existing panels or workflows may be needed due to fix/perm step.|
|Number of samples||Barcode up to 20 samples.|
|Resources||Application Note: The Benefits of Palladium Barcoding on Data Quality and Workflow
Example Cell-ID 20-Plex Barcoding FCS Files
Download Barcoding Key
Cell-ID 20-Plex Pd Barcoding Kit contents (PN 201060)
|3 sets of 20 barcodes in PCR tubes||10 µL each tube|
|Maxpar® 5X Fix I Buffer||15 mL|
|Maxpar Cell Staining Buffer||500 mL|
|Maxpar 10X Barcode Perm Buffer||50 mL|
|Maxpar PBS||500 mL|
- Behbehani, G.K. et al. “Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining.” Cytometry Part A 85 (2014): 1,011–9.
- Bodenmiller, B. et al. “Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators.” Nature Biotechnology 30 (2012): 858–67.
- Zunder, E.R. et al. “Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm.” Nature Protocols 10 (2015): 316–33.
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