Maxpar Antibody Conjugation Service for Mass Cytometry
Expand your CyTOF panels with custom isotope-conjugated antibodies
Mass cytometry is the most comprehensive way to profile cell phenotype and function, enabling simultaneous detection of up to 50 cellular targets in every precious sample.
Whether you’re building a 15- or 50-parameter mass cytometry panel, the extensive Fluidigm catalog of preconjugated antibodies and antibody panel kits will get you there.
We understand your panel design needs. If you have a proprietary antibody to add to your panel or require customized design, our Maxpar® Antibody Conjugation Service is committed to your success.
Select the desired isotope from our 44 high-purity metal tags (including Bi209, Y89, and 7 new cadmium tags), decide which confirmation method you prefer—biochemical analysis or more extensive biologic functional testing—and place your order.
After our expert scientists perform the conjugation and analysis, we’ll send your antibody conjugate along with a detailed analytic report.
Highlights
Flexible
Extend your mass cytometry panels by adding metal-labeled targets not yet available in the Maxpar catalog.
Efficient
Save time and resources by letting the experts do the labeling and quality control for you.
Dependable
Conjugation service performed by our professional technicians ensures product with consistently high quality and reliable performance.
Custom Conjugation Workflow
Custom Conjugation Service Specifications and Ordering Information
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Antibody Conjugation Service with Biological Confirmation |
Antibody Conjugation Service with Biochemical Confirmation |
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| Biochemical analysis provided | Yes | Yes | |
| Biological confirmation performed | Yes (Available for Helios™/CyTOF® only)* |
No | |
| Staining profile and titer data | Yes | Yes | |
| Timeline | 5 to 6 weeks from receipt of antibody | 3 to 4 weeks from receipt of antibody | |
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Yield |
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| Standard scale Input 200 µg Purified, protein-free buffer |
Cat. No. 3999999-1 Yield: 100 tests (1–3 x 106 cells) |
Cat. No. 3999999-2 Yield: 40 µg minimum |
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| Bulk scale Variable input mass of antibody Purified, protein-free buffer |
Cat. No. 3999999-5 Extra 100-test vial Additional units of 100 tests each |
Cat. No. 3999999-7 Yield: 200 µg minimum |
Cat. No. 3999999-8 Yield: 600 µg minimum |
| *Not available for antibodies used on the Hyperion™ Imaging System | |||
Ligands and buffer suitable for custom conjugation—IgG monoclonal and polyclonal antibodies are routinely conjugated. The feasibility of conjugating other antibody isotypes and proteins is considered on a case-by-case basis. To facilitate labeling, ligands must be provided purified, unconjugated and in a protein-free buffer at minimum concentration of 0.5 mg/mL.
Source of antibodies—Antibodies to be custom-conjugated may be either provided by the customer or purchased from a commercial source. Where Fluidigm purchases an antibody on behalf of a customer, the cost is quoted and billed as a separate line item, Cat. No. 3999999-3, Antibody Material Handling Charge.
Quality control—All custom conjugations are subject to rigorous biochemical confirmation, including inductively coupled plasma mass spectrometry, determination of concentration and yield and assessment of ratio of attachment of metal atoms to antibody. Optionally available biological confirmation service involves empirical testing by CyTOF on cells, with optimal titer determined and the conjugate formulated for delivery in a 100-test vial.
Contact us to learn more about Maxpar Custom Antibody Conjugation Services.
Download the Maxpar Custom Antibody Conjugation Services flyer.
Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see www.fluidigm.com/legal/notices.