1000 results found in Biomark
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faq
What is the Advanta IO Gene Expression Assay?
The Advanta™ IO Gene Expression Assay qPCR assay panel enables profiling of tumor immunobiology and identification of new biomarkers. …
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faq
How many genes are in the Advanta IO Gene Expression Assay?
170 genes are assigned to two sets, designated as Panel A and Panel B. …
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faq
What genes are covered by the Advanta IO Gene Expression Assay?
The complete panel set includes genes for identification and functional analysis of immune and cancer cells. …
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faq
What chemistry is used for the Advanta IO Gene Expression Assay?
Thermo Fisher Scientific Applied Biosystems® TaqMan® probes and primers. …
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faq
Is it possible to add assays to the Advanta IO Gene Expression Assay?
Yes, 17 open assay inlets on Panel B are available for additional TaqMan® assays. Assays (at 20X concentration) can be sourced directly from Thermo Fisher Scientific. …
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faq
Do I have to purchase both panels of the Advanta IO Gene Expression Assay?
No, we offer configurations of the Advanta™ IO Gene Expression Assay that support either panel separately or both panels as a set. …
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faq
I already have IFCs. Can I just buy the assays for the Advanta IO Gene Expression Assay?
No, the reagents included in the Advanta™ IO Gene Expression Assay have been analytically validated together to ensure optimal performance across all genes. …
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faq
Which IFC is used for the Advanta IO Gene Expression Assay?
We offer configurations of the Advanta™ IO Gene Expression Assay that support the 96.96 and 24.192 Dynamic Array™ IFCs for Gene Expression (GE 96.96 and GE 24.192 IFCs). …
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faq
What equipment is required to use the Advanta IO Gene Expression Assay?
The Biomark™ HD system and the IFC Controller HX (for 96.96) or RX (for 24.192) or Juno™ system (for both 96.96 and 24.192). …
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faq
What reagents are required to run the Advanta IO Gene Expression Assay?
The assay is available in several configurations. …
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faq
How many samples can be processed in a single run of the Advanta IO Gene Expression Assay?
The number of samples depends on the IFC format and the number of replicates per sample. …
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faq
How many runs can be accomplished in a single day with the Advanta IO Gene Expression Assay?
Two to three runs, depending on operational setup. …
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faq
What is the workflow of the Advanta IO Gene Expression Assay?
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faq
What level of skill is required to perform the Advanta IO Gene Expression Assay (workflow complexity)?
General molecular biology lab techniques, including the use of multichannel pipettes. …
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faq
What level of manual input is required for the Advanta IO Gene Expression Assay?
Approximately 1.3 hours of manual hands-on time is necessary for the assay. …
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faq
Can I automate use of the Advanta IO Gene Expression Assay with my liquid handler?
Yes. Contact techsupport@fluidigm.com to schedule a meeting with your Field Application Scientist for assistance. …
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faq
What additional materials are needed to use the Advanta IO Gene Expression Assay kit?
All necessary reagents, assays, and IFCs are included in the kit. …
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faq
Which software version should I use for the Advanta IO Gene Expression Assay?
Use Biomark™ Data Collection software (version 4.5.1 or higher), which supports use of the GE 24.192 IFC and enables you to select multiple assays (detectors) as reference for differential expression calculations. See the Real-Time PCR Analysis User Guide (PN 68000088) for more information. …
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faq
Which genes should I use as reference with the Advanta IO Gene Expression Assay to normalize expression?
Five reference genes included in both Panel A and Panel B may be used as reference genes. The expression level of genes selected as a reference should be independent of the conditions under investigation. …
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faq
How should I perform data analysis for the Advanta IO Gene Expression Assay?
Use the Real Time PCR Analysis software (version 4.5.1 or higher) to conduct primary data analysis. …