1000 results found in CyTOF 1 & CyTOF 2 and Helios
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technical note
CyTOF 6.7 Data Storage Requirements
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Publications
Application of phospho-CyTOF® to characterize immune activation in patients with sickle cell anemia in an ex vivo model of thrombosis
Sickle cell disease (SCD) is a genetic disease caused by mutations in the beta globin gene, and inflammation plays a key role in driving many aspects of disease pathology. …
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faq
Why is the use of CAS and WB Injector only recommended for Helios? Wouldn’t samples run on any mass cytometer benefit from being resuspended in CAS?
Although we have not rigorously tested the use of Cell Acquisition Solution (CAS) with samples run on CyTOF® 2 systems, it is likely that some benefit to cell signal strength and stability would be seen on those systems as well. …
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Publications
Hepatitis B virus–specific T cells associate with viral control upon nucleos(t)ide-analogue therapy discontinuation
The clinical management of chronic hepatitis B virus (HBV) patients is based exclusively on virological parameters that cannot independently determine in which patients nucleos(t)ide-analogue (NUC) therapy can be safely discontinued …
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Publications
Identification of vaccine-altered circulating B cell phenotypes using mass cytometry and a two-step clustering analysis
Broadening our understanding of the abundance and phenotype of B cell subsets that are induced or perturbed by exogenous Ags will improve the vaccine evaluation process. …
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Publications
In-depth phenotypic characterization of reticulocyte maturation using mass cytometry
Progress towards an in-depth understanding of the final steps of the erythroid lineage development is paramount for many hematological diseases. …
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Publications
Clonal analysis of Salmonella-specific effector T cells reveals serovar-specific and cross-reactive T cell responses
To tackle the complexity of cross-reactive and pathogen-specific T cell responses against related Salmonella serovars, we used mass cytometry, unbiased single-cell cloning, live fluorescence barcoding, and T cell-receptor sequencing to reconstruct the Salmonella-specific repertoire of circulating effector CD4+ T cells, isolated from volunteers challenged with Salmonella enterica serovar Typhi (S. Typhi) or Salmonella Paratyphi A (S. Paratyphi). …
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Publications
Identification and characterization of HIV-specific resident memory CD8+ T cells in human lymphoid tissue
Current paradigms of CD8+ T cell-mediated protection in HIV infection center almost exclusively on studies of peripheral blood, which is thought to provide a window into immune activity at the predominant sites of viral replication in lymphoid tissues (LTs). …
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faq
How is this new cell staining protocol different from previous Fluidigm protocols, and what makes it better?
The biggest change is that the updated protocols all call for use of freshly prepared 1.6% formaldehyde solution. Fluidigm recommends using ampules of Pierce™ 16% (w/v) Formaldehyde, Methanol-free (Thermo Scientific™ Cat. No. 28906, 10 x 1 mL). Other sources of fixative are available. Please contact your field applications scientist for more information. Optimal staining and cell integrity is only obtained using fixative prepared from a new ampule daily. …
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faq
Is the cleaning procedure any different for this new injector?
The procedure is the same. However, the WB Injector needs more frequent cleaning than the HT Injector. …
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faq
What are the new protocols for mass cytometry that I have been hearing about?
Fluidigm has released updated protocols for all cell staining and fixation procedures. The most important update is the use of freshly prepared formaldehyde fixative each day that cells are stained. The updated protocols can be found here. …
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faq
How do I order the new injector? How much does it cost?
The first WB Injector is supplied free of charge along with a supply of Maxpar® Cell Acquisition Solution. Please contact your field application scientist (FAS) if you would like to try out the new Helios™ sample acquisition protocol and your FAS will help you place the order. Additional injectors can be purchased through Fluidigm by referencing Cat. No. 107950. …
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User Guide
Helios User Guide
This guide is intended for users and operators of the Helios™ instrument. The guide provides an introduction to mass cytometry and the Helios system.
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Protocol
Maxpar Nuclear Antigen Staining Protocol Update 2018
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Publications
Bystander CD8+ T cells are abundant and phenotypically distinct in human tumour infiltrates
Various forms of immunotherapy, such as checkpoint blockade immunotherapy, are proving to be effective at restoring T cell-mediated immune responses that can lead to marked and sustained clinical responses, but only in some patients and cancer types. …
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tds
Anti-Human CD66b (80H3)-152Sm
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tds
Anti-Human CD66b (80H3)-162Dy
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Publications
Mass cytometry of Hodgkin lymphoma reveals a CD4+ regulatory T-cell-rich and exhausted T-effector microenvironment
In classical Hodgkin lymphoma (cHL), the host antitumor immune response is ineffective. …
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Publications
Characterization of TRKA signaling in acute myeloid leukemia
Tropomyosin-related kinase A (TRKA) translocations have oncogenic potential and have been found in rare cases of solid tumors. …
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Mass cytometry reveals distinct platelet subtypes in healthy subjects and novel alterations in surface glycoproteins in Glanzmann thrombasthenia
Mass cytometry (MC) uses mass spectrometry to simultaneously detect multiple metal-conjugated antibodies on single cells, thereby enabling the detailed study of cellular function. …