1000 results found in CyTOF 1 & CyTOF 2 and Helios
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Publications
High throughput automated analysis of big flow cytometry data
The rapid expansion of flow cytometry applications has outpaced the functionality of traditional manual analysis tools used to interpret flow cytometry data. …
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Publications
Implementation of Mass Cytometry as a Tool for Mechanism of Action Studies in Inflammatory Bowel Disease
Novel therapeutics for inflammatory bowel disease (IBD) are under development, yet mechanistic readouts at the tissue level are lacking. …
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Publications
Macrophage migration inhibitory factor regulates U1-snRNP immune complex mediated activation of the NLRP3 inflammasome
High expression alleles of macrophage migration inhibitory factor (MIF) are linked genetically to SLE disease severity. …
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Publications
Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia
The absence of cancer-restricted surface markers is a major impediment to antigen-specific immunotherapy using chimeric antigen receptor (CAR) T cells. …
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Publications
T cell-directed IL-17 production by lung granular γδ T cells is coordinated by a novel IL-2 and IL-1β circuit
Immune-mediated lung is considered the result of an exacerbated innate injury immune response, although a role for adaptive lymphocytes is emerging. …
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Publications
High-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia
Aging is associated with tissue-level changes in cellular composition that are correlated with increased susceptibility to disease. …
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Publications
Induction and transcriptional regulation of the co-inhibitory gene module in T cells
The expression of co-inhibitory receptors, such as CTLA-4 and PD-1, on effector T cells is a key mechanism for ensuring immune homeostasis. …
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Publications
Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization
Airway epithelial cells (AECs) secrete innate immune cytokines that regulate adaptive immune effector cells. …
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Publications
T cell cytolytic capacity is independent of initial stimulation strength
How cells respond to myriad stimuli with finite signaling machinery is central to immunology. …
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Publications
Antigen-mediated regulation in monoclonal gammopathies and myeloma
A role for antigen-driven stimulation has been proposed in the pathogenesis of monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM) based largely on the binding properties of monoclonal Ig. …
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tds
Anti-Human PDGFRa (D13C6)-160Gd
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Publications
Functional screening of FGFR4-driven tumorigenesis identifies PI3K/mTOR inhibition as a therapeutic strategy in rhabdomyosarcoma
Rhabdomyosarcoma (RMS) is the most common pediatric soft tissue sarcoma and outcomes have stagnated, highlighting a need for novel therapies. …
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technical note
CyTOF 6.7 Data Storage Requirements
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faq
Why is the use of CAS and WB Injector only recommended for Helios? Wouldn’t samples run on any mass cytometer benefit from being resuspended in CAS?
Although we have not rigorously tested the use of Cell Acquisition Solution (CAS) with samples run on CyTOF® 2 systems, it is likely that some benefit to cell signal strength and stability would be seen on those systems as well. …
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Publications
Hepatitis B virus–specific T cells associate with viral control upon nucleos(t)ide-analogue therapy discontinuation
The clinical management of chronic hepatitis B virus (HBV) patients is based exclusively on virological parameters that cannot independently determine in which patients nucleos(t)ide-analogue (NUC) therapy can be safely discontinued …
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Publications
Identification of vaccine-altered circulating B cell phenotypes using mass cytometry and a two-step clustering analysis
Broadening our understanding of the abundance and phenotype of B cell subsets that are induced or perturbed by exogenous Ags will improve the vaccine evaluation process. …
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Publications
In-depth phenotypic characterization of reticulocyte maturation using mass cytometry
Progress towards an in-depth understanding of the final steps of the erythroid lineage development is paramount for many hematological diseases. …
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Publications
Clonal analysis of Salmonella-specific effector T cells reveals serovar-specific and cross-reactive T cell responses
To tackle the complexity of cross-reactive and pathogen-specific T cell responses against related Salmonella serovars, we used mass cytometry, unbiased single-cell cloning, live fluorescence barcoding, and T cell-receptor sequencing to reconstruct the Salmonella-specific repertoire of circulating effector CD4+ T cells, isolated from volunteers challenged with Salmonella enterica serovar Typhi (S. Typhi) or Salmonella Paratyphi A (S. Paratyphi). …
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Publications
Identification and characterization of HIV-specific resident memory CD8+ T cells in human lymphoid tissue
Current paradigms of CD8+ T cell-mediated protection in HIV infection center almost exclusively on studies of peripheral blood, which is thought to provide a window into immune activity at the predominant sites of viral replication in lymphoid tissues (LTs). …
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faq
How is this new cell staining protocol different from previous Fluidigm protocols, and what makes it better?
The biggest change is that the updated protocols all call for use of freshly prepared 1.6% formaldehyde solution. Fluidigm recommends using ampules of Pierce™ 16% (w/v) Formaldehyde, Methanol-free (Thermo Scientific™ Cat. No. 28906, 10 x 1 mL). Other sources of fixative are available. Please contact your field applications scientist for more information. Optimal staining and cell integrity is only obtained using fixative prepared from a new ampule daily. …