156 results found in Hyperion Imaging System
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Publications
The reconstructed natural history of type 1 diabetes mellitus
The causes of type 1 diabetes mellitus (T1DM) are unclear; however, a general consensus exists that T1DM is a T cell-mediated autoimmune disease characterized by the selective destruction of insulin-secreting β-cells. …
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Publications
Multiplexed (18-Plex) Measurement of Signaling Targets and Cytotoxic T Cells in Trastuzumab-Treated Patients using Imaging Mass Cytometry
Imaging mass cytometry (IMC) uses metal-conjugated antibodies to provide multidimensional, objective measurement of protein targets. …
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Publications
Considerations for immunohistochemistry
Although immunohistochemistry (IHC) has been known and applied for decades, major advances such as robotic sample processing, digital slide image capture, and innovative computerized data analysis uphold the relevance of the technology in current laboratory practice. …
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Publications
The role of proteomics in assessing beta-cell dysfunction and death in type 1 diabetes
Type 1 diabetes (T1D) is characterized by autoimmune-induced dysfunction and destruction of the pancreatic beta cells. …
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Publications
Cross-species single-cell analysis of pancreatic ductal adenocarcinoma reveals antigen-presenting cancer-associated fibroblasts
Cancer-associated fibroblasts (CAFs) are major players in the progression and drug resistance of pancreatic ductal adenocarcinoma (PDAC). …
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Publications
Development of a 2-dimensional atlas of the human kidney with Imaging Mass Cytometry
An incomplete understanding of the biology of the human kidney, including the relative abundances of and interactions between intrinsic and immune cells, has long constrained the development of therapies for kidney disease. …
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Publications
Human lymphoid organ cDC2 and macrophages play complementary roles in T follicular helper responses
CD4+ T follicular helper (Tfh) cells are essential for inducing efficient humoral responses. T helper polarization is classically orientated by dendritic cells (DCs), which are composed of several subpopulations with distinct functions. …
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Publications
Imaging Mass Cytometry and single-cell genomics reveals differential depletion and repletion of B-cell populations following ofatumumab treatment in cynomolgus monkeys
Ofatumumab is the first, fully human, anti-CD20 monoclonal antibody in Phase 3 development for multiple sclerosis (MS). …
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Publications
High-Dimensional Single-Cell Mapping of Central Nervous System Immune Cells Reveals Distinct Myeloid Subsets in Health, Aging, and Disease
Individual reports suggest that the central nervous system (CNS) contains multiple immune cell types with diverse roles in tissue homeostasis, immune defense, and neurological diseases. …
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Publications
Multiplex protein detection on circulating tumor cells from liquid biopsies using Imaging Mass Cytometry
Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. …
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Publications
Compensation of signal spillover in suspension and Imaging Mass Cytometry
The advent of mass cytometry increased the number of parameters measured at the single-cell level while decreasing the extent of crosstalk between channels relative to dye-based flow cytometry. …
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Publications
Immune activation underlies a sustained clinical response to Yttrium-90 radioembolisation in hepatocellular carcinoma
Yttrium-90 (Y90)-radioembolisation (RE) significantly regresses locally advanced hepatocellular carcinoma and delays disease progression. …
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Publications
Ruthenium Counterstaining for Imaging Mass Cytometry
Imaging mass cytometry is a novel imaging modality that enables simultaneous antibody-based detection of more than 40 epitopes and molecules in tissue sections at subcellular resolution using isotopically pure metal tags. …
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Publications
Opposing Roles of Dendritic Cell Subsets in Experimental GN
Dendritic cells (DCs) are thought to form a dendritic network across barrier surfaces and throughout organs, including the kidney, to perform an important sentinel function. …
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Publications
Clickable and High-Sensitivity Metal-Containing Tags for Mass Cytometry
Mass cytometry is a highly multiplexed single-cell analysis platform that uses metal-tagged reagents to identify multiple cellular biomarkers. …
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Publications
Biodistribution of cisplatin revealed by imaging mass cytometry identifies extensive collagen binding in tumor and normal tissues
Imaging mass cytometry was used for direct visualization of platinum localization in tissue sections from tumor and normal tissues of cisplatin-treated mice bearing pancreas cancer patient-derived xenografts. …
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Publications
Analytical figures of merit for a novel tissue imaging system
A novel laser-ablation sample introduction system has been developed for the Fluidigm® Helios™ mass cytometer to enable highly multiplexed imaging of biological samples. …
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Publications
histoCAT: analysis of cell phenotypes and interactions in multiplex image cytometry data
Single-cell, spatially resolved omics analysis of tissues is poised to transform biomedical research and clinical practice. …
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Publications
Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry
To build comprehensive models of cellular states and interactions in normal and diseased tissue, genetic and proteomic information must be extracted with single-cell and spatial resolution. …
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Publications
Mass Spectrometry Imaging and Integration with Other Imaging Modalities for Greater Molecular Understanding of Biological Tissues
Over the last two decades, mass spectrometry imaging (MSI) has been increasingly employed to investigate the spatial distribution of a wide variety of molecules in complex biological samples. …