CyTOF 2 and Helios Operation

CyTOF 2 and Helios OperationDuration: 7 minutes

Sample Capillary Assembly: Heater Bulkhead End

  • Before Beginning

    Before beginning the procedure, please have on hand:

    • Sample Capillary Kit (PN 101519)
    • A clean, dry nebulizer

  • Step 1

    Remove the sample capillary from the kit.

  • Step 2

    Fold the sample capillary in half and cut the sample capillary into two pieces using a clean, sharp razor.

  • Step 3

    Remove the union assembly from the resealable bag. The union assembly contains:

    • 1/16 inch nut
    • 1/8 inch nut

    Remove the following:

    • 1/16 inch nut
    • Blue or clear sleeve
    • Stainless steel back ferrule
    • Yellow back ferrule

  • Step 4

    Remove one sleeve (blue or clear) from the resealable bag and insert one piece of sample capillary into the sleeve.

  • Step 5

    Insert the sample capillary into the 1/16 inch nut.

  • Step 6

    Place the stainless steel back ferrule over the sleeve, with the tapered end of the ferrule facing out.

  • Step 7

    Place the yellow back ferrule over the sleeve, flat side out.

  • Step 8

    Remove the 1/16 inch nut from the union.

  • Step 9

    Use the side of the union to push the yellow back ferrule flush with the sleeve and sample capillary.

  • Step 10

    Carefully insert this assembly into the open end of the union and tighten.

  • Step 11

    Unscrew the union. The sample capillary should be flush with the edge of the yellow back ferrule and sleeve.

  • Step 12

    Verify that the capillary is tight within the assembly and does not slide easily.

    NOTE Do not disturb the flush assembly.