CyTOF 2 Maintenance

CyTOF 2 Maintenance10 minutes

Torch Alignment Check

  • Step 1

    Switch off the RF generator power using the RFG circuit breaker on the right side of the CyTOF® 2.

    Open the front door of the CyTOF and view the positions of the screws in the screw assembly with respect to one another.

  • Step 2

    Compare the amount of thread engagement between the two screw assemblies. Loosen or tighten the corresponding screws to equalize the thread engagement.

  • Step 3

    Tighten the screws in unison to install as required.

    Check the Z-alignment (the position of the Torch relative to the vacuum interface). This ensures that the optimal analytical zone of the plasma is sampled.

  • Step 4

    Install the torch positioning tool (may be either black or silver) into the end of the Torch. Check that it spins freely. Gently push it in until it is flush with the edge of the Torch.

    If the tool is not flush with the Torch (┬▒ 1 mm), inspect the coils to ensure that they are not distorted or damaged. If you have concerns, contact Fluidigm Support.