Sample Barcoding
High-Plex. High-Throughput.
Sample multiplexing by cell barcoding for CyTOF
Sample-specific cell barcoding for simplified workflows and improved staining consistency
Scalable—Combine 35-plus samples at once to run larger experiments.
Flexible—Integrate barcodes with CyTOF panels without compromising panel size.
Powerful—Improve data quality and streamline workflows.
Sample barcoding has been used in flow cytometry workflows for many years. The process tags each cell in a given sample with a unique identifier, enabling sample multiplexing for improved workflows and enhanced data quality.
Only CyTOF® technology enables barcoding workflows for 35 or more samples stained with high-parameter panels of 30 or more markers, providing unique value for use of mass cytometry in translational and clinical research studies.
Use sample barcoding to achieve higher throughput for scaled-up experiments and reduce batch variability, providing even greater data consistency for large studies.
How barcoding works
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Individual samples are barcoded with a unique metal isotope code.
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Barcoded samples are combined into a single, multiplexed sample and stained with the panel of markers.
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The multiplexed sample is acquired on a CyTOF system.
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Data are debarcoded for individual sample analysis.
Barcoding methods
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Palladium-Based Barcoding |
Live-Cell Barcoding |
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Product |
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Cell type |
Universal, cell-agnostic |
Best for human leukocytes, CD45-negative cells excluded |
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Configuration |
Ready-to-use kit and accompanying software |
Individual anti-CD45 antibody selection as required |
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Sample number |
Barcode up to 20 samples. |
Flexible design allows scaling for sample number to 50-plus. |
Webinars
Sample Multiplexing for Cytometry Studies | Roberto Spada, PhD
Resources
Application Notes
Publications
Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see www.fluidigm.com/legal/notices