Enabling multiparameter profiling of precious samples, mass cytometry has been instrumental in achieving new research breakthroughs in areas such as immunology, cancer immunotherapy, drug discovery, vaccine development and more around the world.

Watch the seminars and webinars below to learn how researchers are utilizing Helios™, a CyTOF system, and mass cytometry applications to further their research.

	Systems-Level Immunomonitoring in Human Subjects
	Petter Brodin, MD, PhD Assistant Professor Science for Life Laboratory, Karolinska Institutet, and Department of Neonatology,        Karolinska University Hospital, Stockholm WATCH
	Human Immune Profiling Simplified
	Michelle Poulin, PhD Senior Manager, Field Applications Fluidigm
	Thiru Selvanantham, PhD Senior Product Applications Scientist Fluidigm WATCH
	Unraveling Human Microglial Heterogeneity
	Chotima Böttcher, PhD Senior Scientist and Principle Investigator of Molecular Psychiatry  Charité – University Medicine Berlin WATCH
	Advancing Human Systems Immunology
	Petter Brodin, MD, PhD Assistant Professor Science for Life Laboratory, Karolinska Institutet, and Department of Neonatology,        Karolinska University Hospital, Stockholm WATCH
	Linking T Cell Exhaustion to Disease with Mass Cytometry
	Bertram Bengsch, MD, PhD Group Leader, Department of Internal Medicine II University Medical Center Freiburg WATCH
	Mass Cytometry for Immune Profiling in Chronic Inflammation
	Henrik Mei, PhD Scientific Head of Mass Cytometry DRFZ Berlin, a Leibniz Institute WATCH
	Maximizing Human Immune Monitoring with Mass Cytometry
	Adeeb Rahman  Assistant Professor Icahn School of Medicine at Mount Sinai
	Michelle Poulin, PhD Manager, Field Applications Proteomics Fluidigm WATCH
	Powering Multi-Omic Translational Studies with Mass Cytometry
	Brice Gaudilliere, MD, PhD  Assistant Professor, Department of Anesthesiology Stanford University School of Medicine
	Nima Aghaeepour, PhD Assistant Professor Stanford University School of Medicine WATCH
	Finding Pathologic T Cells in Rheumatoid Arthritis by Mass Cytometry
	Deepak Rao, MD, PhD  Rheumatologist Co-Director, Human Immunology Center Brigham and Women's Hospital WATCH
	Querying Cytokine-Signaling Networks in Myeloproliferative Neoplasms
	Stephen Oh, MD, PhD  Assistant Professor of Medicine, Division of Hematology  Washington University School of Medicine  WATCH
	Enhancing Checkpoint Blockade in Lymphoma with In Situ Vaccination
	Joshua Brody, MD  Assistant Professor, Hematology and Medical Oncology Director, Lymphoma Immunotherapy Program Icahn School of Medicine at Mount Sinai WATCH
	Decoding Tumors with Mass Cytometry
	Jonathan Irish, PhD Assistant Professor of Cancer Biology, Assistant Professor of Pathology, Microbiology and Immunology Vanderbilt University WATCH
	Defining the Phenotypic and Functional Immune Microenvironment in AML
	Evan Lind, PhD Assistant Professor, Department of Molecular Microbiology and Immunology and Department of Cell, Development and Cancer Biology Oregon Health & Science University WATCH
	Towards an Assay of Global Immune Competence
	Holden Maecker, PhD Associate Professor (Research) of Microbiology and Director, Human Immune Monitoring Center Stanford University WATCH
	Signatures of Protective NK Cell Responses
	Catherine Blish, MD, PhD Assistant Professor, Department of Medicine and Immunology, Stanford University School of Medicine Assistant Director of the Stanford Medical Scientist Training Program WATCH

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Immune profiling of PBMC and peripheral whole blood is an essential method for quantifying changes in immune cell populations in autoimmune disease, chronic inflammation, infectious disease and cancer.

In this webinar, Michelle Poulin, Manager, Field Applications, introduces an innovative mass cytometry application, the Maxpar® Direct™ Immune Profiling System, which provides a complete sample-to-answer solution for high-parameter immune monitoring.

The system combines

• CyTOF^® technology on the Helios™ system
• A dry, single-tube 30-marker antibody panel
• Automated Maxpar Pathsetter™ software.

It enables enumeration of 37 immune cell populations from PBMC and whole blood in as little as 5 minutes. The system is also flexible, allowing for addition of antibodies and development of new analysis models.

Thiru Selvanantham, Senior Product Applications Scientist, presents data on repeatability, precision and accuracy, as well as site-to-site reproducibility from external multisite testing of the system.

	Michelle Poulin, PhD Manager, Field Applications Fluidigm
	Thiru Selvanantham, PhD Senior Product Applications Scientist Fluidigm

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Fluidigm Products are for Research Use Only. Not for use in diagnostic procedures.

Systems-Level Immunomonitoring in Human Subjects

In this recorded seminar Dr. Petter Brodin presents his most recent data on the application of systems-level immune monitoring using mass cytometry in a variety of clinical studies. He discusses his lab’s innovative approaches to tackling the challenges of longitudinal sampling and analysis of whole blood samples for immune monitoring as well as the biologic results obtained from several studies.

Watch this seminar to learn about:

• Brodin’s approach to reducing technical variation introduced during sample processing and data analysis.
• Results from three ongoing immune monitoring projects:
• Immune variation in a cohort of normal adults
• A pediatric oncology clinical study to comprehensively monitor immune system impact of treatment across a variety of cancers
• The evolution of the human newborn immune system

Petter Brodin, MD, PhD Associate Professor of Immunology SciLifeLab, Department of Women's and Children's Health Department of Newborn Medicine Karolinska University Hospital Karolinska Institutet

Recorded at the 8th Annual Fluidigm Mass Cytometry Summit June 20, 2019

For Research Use Only. Not for use in diagnostic procedures.

Watch these short video interviews from scientists who have been using Imaging Mass Cytometry™ to gain research insights.

Learn more about Imaging Mass Cytometry.

Interviews

Frits Koning, PhD
Professor
Department of Immunohematology and Blood Transfusion
Leiden University Medical Center

Jen Gommerman, PhD
Professor and Associate Chair, Graduate Studies
Department of Immunology
University of Toronto

Tom Ashhurst, BSc
High-Dimensional Cytometry Specialist
Sydney Cytometry Facility
University of Sydney

Stephen Oh, MD, PhD
Assistant Professor
Department of Medicine, Hematology Division
Washington University School of Medicine in St. Louis

Akil Merchant, MD
Assistant Professor of Medicine
Keck School of Medicine
University of Southern California

Want to learn more about Imaging Mass Cytometry? Leave us your information so we can include you on future IMC™ communications.

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Fluidigm Products are for Research Use Only. Not for use in diagnostic procedures.

Seminars and Webinars

Investigating cellular interrelationships in spatial context helps researchers illuminate changes that may correlate with disease progression and therapeutic response.

Watch these seminars to learn how researchers are using the Hyperion™ Imaging System for tissue microenvironment characterization, biomarker discovery and more.

	Imagining the Unimaginable with Imaging Mass Cytometry Frits Koning, PhD Professor Department of Immunohematology and Blood Transfusion Leiden University Medical Center WATCH
	Using IMC to Better Understand Mechanisms of MS Disease Progression Jen Gommerman, PhD Professor and Associate Chair, Graduate Studies Department of Immunology University of Toronto WATCH
	Understanding the Immune Composition and Therapeutic Vulnerabilities of Human Lung Cancer Using Imaging Mass Cytometry Kurt Schalper, MD, PhD Assistant Professor of Pathology Director, Translational Immuno-Oncology Laboratory Yale Cancer Center WATCH
	Uncovering Cellular Social Networks: Analysis of Tissue Ecosystems in Health and Disease by Imaging Mass Cytometry Bernd Bodenmiller, PhD  Assistant Professor, Institute of Molecular Life Sciences University of Zurich WATCH
	Advancing Translational and Clinical Research: Imaging Mass Cytometry to Characterize the Tumor Immune Microenvironment Akil Merchant, MD  Assistant Professor of Medicine Keck School of Medicine University of Southern California WATCH

For Research Use Only. Not for use in diagnostic procedures.

The Maxpar® Direct™ Immune Profiling System is the first complete sample-to-answer solution for high-dimensional immune profiling of human PBMC and whole blood.

In this recording, Michelle Poulin, PhD, describes the system which brings together CyTOF® technology on the Helios™ system, a dry 30-marker antibody panel and automated Maxpar Pathsetter™ software so you can easily count 37 immune cell populations with performance you can trust.

Michelle Poulin, PhD Senior Manager, Field Applications  Fluidigm

For Research Use Only. Not for use in diagnostic procedures.

New technical advancements coupled with immune profiling by mass cytometry provide insights into chronic inflammation

In support of research at DRFZ Berlin on immune pathogenesis in chronic inflammation and autoimmunity, Dr. Henrik Mei’s lab at the DRFZ Berlin have developed several technical improvements that enabled generation of high-quality mass cytometry data. Mei describes these innovations in this presentation given at the 7th Annual Mass Cytometry Summit in Prague.

In this live recorded video, Mei presents:

• the development of dual fluorescent/metal-tagged antibodies that permit direct cross-platform comparisons
• the use of silver nanoparticles for sensitive detection of weakly expressed antigens
• the stabilization of cocktails of metal-labeled antibodies for longitudinal or multicenter immune phenotyping studies
• the results of a systematic comparison between blood cells of rheumatoid arthritis subjects and matched controls

Henrik Mei, PhD Scientific Head of Mass Cytometry DRFZ Berlin, a Leibniz Institute

Don't miss out on the 8th Annual Mass Cytometry Summit, taking place from June 20-21, 2019 in Vancouver, Canada.

Register now

References

Baumgart, S. et al. "Dual-labelled antibodies for flow and mass cytometry: a new tool for cross-platform comparison and enrichment of target cells for mass cytometry." European Journal of Immunology 47 (2017): 1,377–1,385.

Schulz, A.R. et al. “Silver nanoparticles for the detection of cell surface antigens in mass cytometry.” Cytometry A 91 (2017): 25–33.

For Research Use Only. Not for use in diagnostic procedures.

Disease-specific features of exhausted CD8 T cells revealed through epigenomic-guided mass cytometry

In this talk from the 7th Annual Mass Cytometry Summit in Prague, Dr. Bertram Bengsch from the University Medical Center Freiburg shares his latest research (Bengsch et al.) on using exhaustion-specific genes found in mice and humans to profile exhausted T cells (TEX) by mass cytometry in patients with HIV and lung cancer.

Hear Bengsch discuss:

• combining an exhaustion severity metric with high-dimensional phenotypes to define TEX clusters shared across chronic infection and cancer or enriched in either disease
• how mass cytometry led to the identification of at least nine distinct TEX clusters in HIV and lung cancer by phenotypic, functional and transcription factor and inhibitory receptor co-expression patterns
• identification of combinatorial patterns of immunotherapy targets on different TEX clusters.

Bertram Bengsch, MD, PhD Group Leader, Department of Internal Medicine II University Medical Center Freiburg

Don't miss out on the 8th Annual Mass Cytometry Summit, taking place from June 20-21, 2019 in Vancouver, Canada.

Register now

References

Bengsch, B. et al. “Epigenomic-guided mass cytometry profiling reveals disease-specific features of exhausted CD8 T cells.” Immunity 48 (2018): 1,029–1,045.e5.

For Research Use Only. Not for use in diagnostic procedures.

Multiplexed Mass Cytometry Analysis of Human Microglia

Hear Chotima Böttcher, PhD, describe her investigation into the diversity and regional distribution of microglia, a specialized innate immune cell in the brain involved in neural development and neuropathology response.

In this talk recorded at the 7th Annual Mass Cytometry Summit in Prague, Böttcher explains how her lab overcame the limitations of studying human microglia and were able to resolve the heterogeneity of this unique cell, previously only demonstrated in the mouse brain.

Watch this video to hear about:

• high-dimensional, large-scale immunophenotyping of post-mortem brain tissue with CyTOF^® technology
• development of a novel cryopreservation protocol in combination with barcoding for single-cell profiling of human microglia
• identification of the phenotypic signature of human microglia in five different brain regions
• comparative analysis of microglia with peripheral myeloid cells and cerebral spinal fluid

Chotima Böttcher, PhD Senior Scientist and Principle Investigator Laboratory of Molecular Psychiatry Charité, University Medicine Berlin

Don't miss out on the 8th Annual Fluidigm Mass Cytometry Summit, taking place from June 20-21, 2019 in Vancouver, Canada.

Register now

References

Böttcher, C. et al. “Human microglia regional heterogeneity and phenotypes determined by multiplexed single-cell mass cytometry.” Nature Neuroscience 22 (2018): 78–90.

For Research Use Only. Not for use in diagnostic procedures.

CyTOF^® and Proseek Analysis of Newborn Blood Provides Novel Insights into Immune System Development

In this presentation, recorded at the 7th Annual Mass Cytometry Summit in Prague, Dr. Petter Brodin of the Karolinska Institutet and Karolinska University Hospital in Stockholm shares his most recent research (Olin, A. et al. 2018) on stereotypic immune system development in newborn children.

Interested in understanding the factors that drive human immune variation, Brodin’s group used mass cytometry and plasma protein analysis to perform a systems-level, longitudinal analysis of blood immune cells and serum after birth.

Hear Brodin discuss:

• technologies developed at his lab enabling analysis of small-volume blood samples
• integration of mass cytometry data and Proseek (Olink®) plasma protein data with clinical information using a topological parameter landscaping method
• how mass cytometry combined with other -omics approaches provided unique insights into the interplay between the environment and the immune system
• the surprising result that the immune systems of children of different gestational ages and environmental conditions still converge on a shared developmental trajectory

Petter Brodin, MD, PhD Assistant Professor, Science for Life Laboratory Karolinska Institutet and Department of Neonatology Karolinska University Hospital, Stockholm

Don't miss out on the 8th Annual Mass Cytometry Summit, taking place from June 20-21, 2019 in Vancouver, Canada.

Register now

References

Olin, A., Henckel, E., Chen, Y. et al. “Stereotypic immune system development in newborn children.” Cell 174 (2018): 1,277–92.

Kaczorowski, K.J., Shekhar, K., Nkulikiyimfura, D. et al. “Continuous immunotypes describe human immune variation and predict diverse responses.” Proceedings of the National Academy of Sciences of the United States of America 114 (2017): E6097–106.

For Research Use Only. Not for use in diagnostic procedures.