Products & Services Instruments Hyperion

Hyperion Imaging System

Visualize the complex spatial biology of the tissue microenvironment with Imaging Mass Cytometry

Gain unprecedented single-cell insights into the intricate spatial relationships of cells, pathways and phenotypes in the tissue microenvironment in the context of disease.

Large image of Fluidigm's Hyperion instrument

Technology

Imaging Mass Cytometry™ (IMC™) with the Hyperion™ Imaging System allows the generation of novel research hypotheses by surveying a high number of protein markers in one scan, saving precious sample, while employing proven CyTOF® technology. A precisely directed laser beam focused at 1 μm collects biological samples stained with metal-tagged Maxpar® antibodies from a region of interest. Discrete signals from each ionized metal tag are detected based on differences in mass instead of wavelength. Thus, IMC eliminates autofluorescence and achieves uniform staining with minimal background. Large panels can be designed with minimal concern about antibody order or label assignment. The Hyperion Imaging System is the ideal solution when you work with heterogeneous biology where assessing key spatial information is critical to driving a deeper understanding of healthy and disease states. Use the system to gain a better understanding of immunotherapy responses that could ultimately help extend patient lives.

Workflow

1

Design

Design panels using pathologist-verified Maxpar antibodies conjugated to metal tags. 

2

Stain

Stain tissues (FFPE or frozen) using familiar immunohistochemistry protocols.

3

Image

Image up to 40 protein markers in one scan at single-cell resolution using the Hyperion Imaging System.

4

Analyze

Analyze images in minutes using the MCD™ Viewer and easily export the resulting files for secondary analysis.

Publications

Join the hundreds of researchers who have gained deep spatial biology insights IMC to phenotype, stratify, discover biomarkers and gain therapeutic insights that address significant translational and clinical research questions. 

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CyTOF Software v7.0

CyTOF® Software v7.0 offers support for the imaging and cell suspension-based sample analysis to Hyperion™ Imaging System and Helios™ system users. The software has the built-in capability to switc...

CyTOF Software (Non-acquisition workstations only)

CyTOF® Software v7.0 offers support for imaging analysis to Hyperion™ Imaging System users.

CyTOF Software v8.0

CyTOF® Software v8.0 offers support for cell suspension-based sample analysis to the CyTOF XT system. The software has the built-in capability for automated calibration, batch sample acquisition, a...

histoCAT

The histoCAT™ software is an innovative computational Imaging Mass Cytometry™ analysis toolbox that enables comprehensive analysis of cellular phenotypes and their interrelationships within the spa...

Indica Labs Software

The Indica Labs Highplex FL HALO® module combines ease of use and scalability with powerful analytics for streamlined biomarker analysis of highly multiplexed Imaging Mass Cytometry™ data. This mod...

MCD Viewer

MCD™ Viewer is post-acquisition data processing software that allows users to visualize, review, and export Imaging Mass Cytometry™ data acquired with the Hyperion™ Imaging System and CyTOF® Softwa...

Visiopharm Software

As high-plex IMC™ data continues to stretch our abilities to interrogate the tumor microenvironment, finding meaningful differences in your data becomes an ever more data-heavy and daunting challen...

Software

Learn about the Imaging Mass Cytometry data analysis pipeline.

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Support

A critical success factor when adopting high-performance technology is acquiring the proficiency and support necessary to accelerate your research and discovery initiatives. Fluidigm PRO Services are a comprehensive portfolio of solutions to help you maximize your investment, scale up productivity and empower your laboratory with greater access to professional resources.

Products

Related products that will help you make the most of this instrument:

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Specifications

The Hyperion Imaging System comprises a Hyperion Tissue Imager, a Helios system, and software for data acquisition and viewing. 

Hyperion Tissue Imager dimensions*

Width

56 cm (22 in)

Height

134 cm (53 in

Depth

56 cm (22 in)

Weight

159 kg (350 lb)

Helios dimensions

Width

103 cm (41 in)

Height

132 cm (52 in)

Depth

87 cm (35 in)

Weight

320 kg (705 lb)

Chiller size

Width

38 cm (15 in)

Height

64 cm (25 in)

Depth

67 cm (27 in)

Weight

81 kg (178 lb)

Hyperion Imaging System

Channels

135

Mass range

75–209 amu

Abundance sensitivity

0.3% for 159Tb

Frequency

200 pixels/sec

Detection limit

≥400 copies per μm2

Dynamic range

4 orders of magnitude

Calibration

Automated

Operating system

Windows® 10 Enterprise

Data storage

OS: 240 GM and Storage: 8 TB

Switch time (between modes)

12 hr

Cross-cell contamination (redeposition)

≤2% (selected direction)

Crosstalk pixel to pixel

≤15% at 200 pixels/sec

Wet tissue thickness for full ablation

≤7 μm

Addressable sample size on slide

≥15 mm x 45 mm

Optical view of sample

≥250 μm x 250 μm

File type

TXT, multipage TIFF, OME-TIFF, MCD

Scan area

≥1 mm2/2 hr (@200 Hz)

Workstation

Operating system

Windows 10 Enterprise

Data storage

OS: 240 GB, Storage: 8 TB

CPU

Intel® Core i7

Memory

32 GB

Dimensions

Height 34.5 cm (14 in) , Width 13.4 cm (5 in), Depth 35.6 cm (14 in), Weight 8 kg (18 lb)

Monitor

86.3 cm (34 in)

Data file size**  

MCD

4–5 MB/channel

TXT

6–8 MB/channel

* The Hyperion Tissue Imager must be placed directly in front of the Helios™

** 1,000 x 1,000 μm region of interest (ROI)

Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see www.fluidigm.com/legal/notices