Juno
Increase productivity and efficiency with automated, cost-effective and easy-to-use workflows for targeted DNA next-generation sequencing (NGS) library preparation.
Using Juno™ you can produce dozens to hundreds of sample libraries daily for hypothesis-free RNA-seq or targeted DNA NGS. Optimized for use with Illumina® sequencing systems in combination with Fluidigm supplied sample barcodes, Juno enables accurate sequencing of more samples—faster and more affordably than ever before.
Technology
Juno integrates IFC control and thermal cycling to enable scalable, automated NGS library preparation for Illumina sequencers and IFC preparation for gene expression, genotyping and digital PCR applications using the Fluidigm Biomark HD.
Juno: NGS Library Preparation Workflow
1
Transfer
Dispense the purified DNA, sample barcodes, PCR primers and master mix into designated inlets on the IFC before placing it onto the Juno loading tray.
2
Run
Press Start. Juno automatically mixes nanoscale reactions within the IFC to amplify DNA targets and barcode sample libraries.
3
Sequence
Targeted DNA sequencing libraries are collected from the IFC, and adapter PCR and cleanup are performed in a single tube reaction, followed by sequencing on an Illumina system.
Juno: Gene Expression Workflow
1
Transfer
Dispense purified cDNA from RNA or miRNAs, qPCR assays and master mix into designated inlets on the IFC before placing it onto the Juno loading tray.
2
Load
Press Start. Juno automatically loads the IFC with input sample, assays and reagents to prepare for thousands of nanoscale real-time PCR reactions.
3
Run\Read
Transfer the IFC with pre-mixed real-time PCR reactions to the Biomark HD for amplification and real-time quantitative detection.
Juno: SNP Genotyping Workflow
1
Transfer
Dispense the purified DNA, sample barcodes, SNP Type™ or probe-based assays and master mix into designated inlets on the IFC before placing it onto the Juno loading tray.
2
Run
Press Start. Juno automatically mixes nanoscale PCR reactions within the IFC and performs amplification using the integrated thermocycler. For compatibility with low sample inputs down to 5.5 ng, the option for a low-cycle-number preamplification exists. This option is fully integrated and automated on the IFC.
3
Read
Transfer the IFC with completed SNP reactions to the Biomark HD or EP1™ for endpoint genotyping analysis.
Publications
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Advanta RNA-Seq Library Normalization Workbook
This custom Microsoft® Excel® workbook is designed for use with the Advanta™ RNA-Seq NGS Library Prep Kit and Advanta RNA-Seq XT NGS Library Prep Kits with the Juno™ system to automate calculations...
Juno Software
The latest software for the Juno™ system. To install, follow the instructions in the Juno System User Guide (100-7070).
Support
A critical success factor when adopting high-performance technology is acquiring the proficiency and support necessary to accelerate your research and discovery initiatives. Fluidigm PRO Services are a comprehensive portfolio of solutions to help you maximize your investment, scale up productivity and empower your laboratory with greater access to professional resources.
Juno™ SNP Type™ Genotyping Reagent Kit—10 IFCs
Reagents for 10 Juno 96.96 Genotyping IFCs using SNP Type assays.Kit contains:1 tube of Juno GT Preamp Master Mix, 1.35 mL2 tubes of SNP Type GT Master Mix, 1.6 mL1 tube of Dilution Reagent, 1.7 mL2 bottles of Dilution Reagent, 3.7 mL2 tubes of 60X SNP Type Reagent, 70 µL1 tube of Juno GT Flux Fluid, 1.0 mLIFCs and Control Line Fluid syringes sold separately. Product insert available.
Advanta™ NGS Library Prep Reagent Kit—LP 192.24, 10 IFCs
This kit contains sufficient reagents to support library preparation with 10 Juno™ LP 192.24 IFCs.Kit contains:1 tube of TSP Sample Loading Reagent V2, 600 µL1 tube of TSP Adapter Mix, 120 µL2 tubes of PCR Water, 1.8 mL1 bottle of C1 DNA Dilution Reagent, 3.0 mL1 bottle of TSP Harvest Reagent, 10 mL1 tube of TSP Assay Loading Reagent, 75 µL5 tubes of 4X TSP Master Mix, 0.63 mL10 tubes of TSP DNA Polymerase, 50 µLIFCs and Control Line Fluid syringes sold separately.
Targeted DNA Seq Library Adapter Set
Unique adapters to enable dual indexing. Each adapter is sufficient for (40) 192.24 LP IFCs or (40) LP 48.48 IFCsKit contains:1 tube of Dual Index Adapter Mix 1, 120 µL1 tube of Dual Index Adapter Mix 2, 120 µL1 tube of Dual Index Adapter Mix 3, 120 µL1 tube of Dual Index Adapter Mix 4, 120 µLIFCs, Control Line Fluid syringes, reagents, and barcodes sold separately.
Resources
Flyers & brochures
Technical documents
Protocols
Quick references
Release notes
Specifications
Instrument
Thermal control
Peltier-based, ranging from 4 °C to 99 °C Maximum heating rate: >4 °C/sec Maximum cooling rate: >3 °C/sec
Depth
69.8 cm (27.5 in)
Width
43.9 cm (17.3 in)
Height
50 cm (19.7 in)
Weight
52 kg (115 lb)
Voltage
100–240 V AC
Frequency
50–60 Hz
Maximum current
8.0 A
Typical average power consumption
Idle: 40 W Operating: 175 W
Work Environment (indoor use only)
Minimum rear clearance
10 cm (4 in)
Minimum front clearance
25 cm (10 in)
Ambient temperature
15–28 ºC (59–82 ºF)
Humidity
20–80%, noncondensing
Pollution degree
2
Electrical installation
Category II
Altitude
Up to 2,000 m (6,562 ft)
IFC Compatibility
Genotyping
Juno 96.96 GT IFC 96.96 GT IFC. 48.48 GT IFC. 192.24 GT IFC Flex Six™ GT IFC
Gene Expression
96.96 GE IFC 48.48 GE IFC 192.24 GE IFC 24.192 GE IFC Flex Six GE IFC
Digital PCR
12.765 dPCR IFC 48.770 dPCR IFC qdPCR 37K™ IFC
Targeted Sequencing
LP 48.48 IFC LP 192.24 IFC
Data Storage
Ethernet connection
1 GB/sec
Hard drive
40 GB
Port
USB port
Computer
Internal CPU with touchscreen interface
Software
System
Juno™ software v3.9.1 or later
Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see www.fluidigm.com/legal/notices