Increase productivity and efficiency with automated, cost-effective and easy-to-use workflows for targeted DNA next-generation sequencing (NGS) library preparation. 

Using Juno™ you can produce dozens to hundreds of sample libraries daily for hypothesis-free RNA-seq or targeted DNA NGS. Optimized for use with Illumina® sequencing systems in combination with Fluidigm supplied sample barcodes, Juno enables accurate sequencing of more samples—faster and more affordably than ever before. 

Large image of Fluidigm's Juno instrument

Technology

Juno integrates IFC control and thermal cycling to enable scalable, automated NGS library preparation for Illumina  sequencers and IFC preparation for gene expression, genotyping and digital PCR applications using the Fluidigm  Biomark HD. 

Juno: NGS Library Preparation Workflow

1

Transfer

Dispense the purified DNA, sample barcodes, PCR primers and master mix into designated inlets on the IFC before placing it onto the Juno loading tray. 

2

Run

Press Start. Juno automatically mixes nanoscale reactions within the IFC to amplify DNA targets and barcode sample libraries. 

3

Sequence

Targeted DNA sequencing libraries are collected from the IFC, and adapter PCR and cleanup are performed in a single tube reaction, followed by sequencing on an Illumina system. 

Juno: Gene Expression Workflow

1

Transfer

Dispense purified cDNA from RNA or miRNAs, qPCR assays and master mix into designated inlets on the IFC before placing it onto the Juno loading tray. 

2

Load

Press Start. Juno automatically loads the IFC with input sample, assays and reagents to prepare for thousands of nanoscale real-time PCR reactions. 

3

Run\Read

Transfer the IFC with pre-mixed real-time PCR reactions to the Biomark HD for amplification and real-time quantitative detection. 

Juno: SNP Genotyping Workflow

1

Transfer

Dispense the purified DNA, sample barcodes, SNP Type™ or probe-based assays and master mix into designated inlets on the IFC before placing it onto the Juno loading tray. 

2

Run

Press Start. Juno automatically mixes nanoscale PCR reactions within the IFC and performs amplification using the integrated thermocycler. For compatibility with low sample inputs down to 5.5 ng, the option for a low-cycle-number preamplification exists. This option is fully integrated and automated on the IFC. 

3

Read

Transfer the IFC with completed SNP reactions to the Biomark HD or EP1™ for endpoint genotyping analysis. 

Advanta RNA-Seq Library Normalization Workbook

This custom Microsoft® Excel® workbook is designed for use with the Advanta™ RNA-Seq NGS Library Prep Kit and Advanta RNA-Seq XT NGS Library Prep Kits with the Juno™ system to automate calculations...

Juno Software

The latest software for the Juno™ system. To install, follow the instructions in the Juno System User Guide (100-7070).

Software

Get the precise results your research deserves!

VIEW ALL

Support

A critical success factor when adopting high-performance technology is acquiring the proficiency and support necessary to accelerate your research and discovery initiatives. Fluidigm PRO Services are a comprehensive portfolio of solutions to help you maximize your investment, scale up productivity and empower your laboratory with greater access to professional resources.

Products

Related products that will help you make the most of this instrument: 

VIEW ALL

Specifications

Instrument

Thermal control

Peltier-based, ranging from 4 °C to 99 °C Maximum heating rate: >4 °C/sec Maximum cooling rate: >3 °C/sec

Depth

69.8 cm (27.5 in)

Width

43.9 cm (17.3 in)

Height

50 cm (19.7 in)

Weight

52 kg (115 lb)

Voltage

100–240 V AC

Frequency

50–60 Hz

Maximum current

8.0 A

Typical average power consumption

Idle: 40 W Operating: 175 W

Work Environment (indoor use only) 

Minimum rear clearance

10 cm (4 in)

Minimum front clearance

25 cm (10 in)

Ambient temperature

15–28 ºC (59–82 ºF)

Humidity

20–80%, noncondensing

Pollution degree

2

Electrical installation

Category II

Altitude

Up to 2,000 m (6,562 ft)

IFC Compatibility 

Genotyping

Juno 96.96 GT IFC 96.96 GT IFC. 48.48 GT IFC. 192.24 GT IFC Flex Six™ GT IFC

Gene Expression

96.96 GE IFC 48.48 GE IFC 192.24 GE IFC 24.192 GE IFC Flex Six GE IFC

Digital PCR

12.765 dPCR IFC 48.770 dPCR IFC qdPCR 37K™ IFC

Targeted Sequencing

LP 48.48 IFC LP 192.24 IFC

Data Storage 

Ethernet connection

1 GB/sec

Hard drive

40 GB

Port

USB port

Computer

Internal CPU with touchscreen interface

Software 

System

Juno™ software v3.9.1 or later

Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see www.fluidigm.com/legal/notices