Access Array

Superior library preparation on the widest scale with a streamlined workflow and low costs

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Amp Up Your Targets

Successful targeted sequencing depends on quality library preparation, and successful prep depends on the Access Array™ system. Combining the high throughput and low cost benefits of microfluidics with unparalleled PCR performance, the Access Array provides superior quality amplicons for SNP identification, sequence variation and mutation detection across hundreds of samples, taking your DNA from sample to sequence-ready in no time.

Specific

Enjoy high-quality results with 95% of amplicons mapped to targets

Sensitive

Get the results you want from only 50 ng of input DNA

Accurate

Reduce errors with a streamlined workflow and only 20 minutes of hands-on time

Compatible

Work on all major next-generation sequencing instruments, like the HiSeq™, MiSeq™ and Ion PGM™

Applications

Technology

Through a combination of Fluidigm’s exclusive integrated fluidic circuits (IFCs) and controllers, the Access Array creates amplicon libraries using a unique tagging protocol, in which primers attach sample-specific barcode sequences and sequencer-specific tags to each PCR product. Multiplex primers build libraries of 480 amplicons per sample, and barcode sequences let you run up to 384 samples in one multiplex sequencing run, delivering the most efficient and accurate library preparation available.

Workflow

  1. Prime
  2. Transfer
  3. Load
  4. Amplify
  5. Harvest

Prime

Prime the Access Array IFC to prepare it for sample transfer.

Transfer

Pipette samples and primer sets into the designated inlets on the IFC.

Load

Automatically combine samples and primers into 2,304 unique reactions in the AX Controller.

Amplify

Insert the IFC in the FC1™ for target amplification.

Harvest

Place the IFC in the IFC Controller AX to collect and purify the amplified products for clonal amplification and sequencing.

components

IFCs, Assays and Reagents

Access Array IFC

Achieve 2,304 unique PCR reactions from 48 samples, and recover your sequence-ready PCR products, with the innovative Access Array™ IFC.

D3 Assay Design

Simplify your assay design with our D3™ tool, which enables you to easily define parameters for customized gene expression, genotyping and targeted DNA sequencing.

Access Array Assays

Maximize sequencing output by targeted library preparation with our high-quality locus-specific primers.

  1. Access Array IFC
  2. D3 Assay Design
  3. Access Array Assays

Related Systems

FC1 Cycler

FC1 Cycler Optimize thermal cycling of your Access Array, Dynamic Array and Digital Array IFCs with the specially designed, scalable and intuitive FC1 cycler.

IFC Controller

IFC Controller Benefit from fewer steps and automated setup of our Access Array IFCs with Fluidigm’s fully integrated IFC Controller AX.
  1. FC1 Cycler
  2. IFC Controller

Software

Access Array Script Update

As part of our continued drive to provide the best-quality products, we have changed the IFC Controller AX software script that manages the harvest of solution.
  1. Access Array Script Update

Specifications

Sequence Captures

Capture per Sample Capture Per Array
Amplicon Tagging* 24kb 1.1MB
Multiplexed Amplicon Tagging* 240kb 11MB
Long Range PCR 480kb 23MB

IFC Controller AX

Dimensions
Height 13 in (33 cm)
Width 9.5 in (24.1 cm)
Depth 19 in (48.3 cm)
Weight 59.4 lb (27 kg)
Environment
Room temperature 20–30 ºC
Relative humidity 30%–80%, non-condensing
Altitude up to 2000 M
Electrical
Installation Category II
Input connector IEC 320-C13
Service 15 A
Input power 500 W maximum
Input voltage 100–240 VAC
Line frequency 50–60 Hz

FC1 Cycler

Dimensions
Height 8 in (20 cm)
Width 9 in (23 cm)
Depth 19 in (48 cm)
Weight 25 lb (11 kg)
Environment
Room temperature 15–30 ºC
Relative humidity 30%–70% non-condensing
Altitude up to 2000 M
Electrical
Installation Category II
Input connector IEC 320-C13
Service 15 A
Input power 200 W maximum
Input voltage 100–240 VAC
Line frequency 50–60 Hz