Access Array

A modular, flexible targeted DNA sequencing library preparation system

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Targeted DNA NGS library preparation

Automate amplicon-based library preparation with the Access Array™ system for up to 48 samples per processing run. Access Array generates next-generation sequencing (NGS)-ready libraries compatible with multiple sequencing platforms including Illumina®, Ion Torrent™, and other NGS systems.

Multiple chemistry options are enabled on Access Array, including Targeted DNA Seq Library Preparation reagents and Access Array reagents.

Specific

Enjoy high-quality results with 95% of amplicons mapped to targets.

Sensitive

Get the results you want from only 50 ng of input DNA.

Accurate

Reduce errors with a streamlined workflow and only 20 minutes of hands-on time.

Compatible

Work on all major
next-generation sequencing instruments, like the HiSeq®, MiSeq™ and Ion PGM™.

Technology

Through a combination of Fluidigm’s exclusive integrated fluidic circuits (IFCs) and controllers, Access Array creates amplicon libraries using a unique tagging protocol, in which primers attach sample-specific barcode sequences and sequencer-specific tags to each PCR product. Multiplex primers build libraries for a few user defined genes to more comprehensive panels of up to 4,800 amplicons from hundreds of genes, and barcode sequences let you run up to 384 samples in one multiplex sequencing run.

Workflow

  1. Prime
  2. Transfer
  3. Load
  4. Amplify
  5. Harvest

Prime

Prime the Access Array IFC to prepare it for sample transfer.

Transfer

Pipet samples and primer sets into the designated inlets on the IFC.

Load

Samples and assays primers are automatically combined in the AX Controller.

Amplify

Insert the IFC in the FC1™ cycler for target amplification.

Harvest

Place the IFC in the IFC Controller AX to collect and purify the amplified products for clonal amplification and sequencing.

Support

Access Array Support

Explore tutorials and publications, browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here. 

components

IFCs, Assays and Reagents

Library Prep 48.48 IFC

Achieve sequencer-ready targeted DNA sample libraries for 48 samples per processing batch with the Library Prep 48.48 IFC.

D3 Assay Design

Simplify your assay design with our D3™ tool, which enables you to easily define parameters for customized targeted DNA sequencing, gene expression and genotyping. For species not supported online, support is available from the D3 expert design team.

Targeted DNA Sequencing and Access Array Assays

Design customized, proprietary panels with ease to screen from hundreds to thousands of genomic variants. Maximize your sequencing output with multiplexed targeted amplicon-library preparation assays. Each primer set delivers a high degree of specificity and uniform coverage of your loci of interest. Choose between Access Array™ assays that are created exclusively for the Access Array IFC (integrated fluidic circuit) and Targeted DNA Seq Library preparation assays designed for use with Juno™ LP 192.24 IFC or LP 48.48 IFC.

  1. Library Prep 48.48 IFC
  2. D3 Assay Design
  3. Targeted DNA Sequencing and Access Array Assays

Related Systems

FC1 Cycler

FC1 Cycler Optimize thermal cycling of your Access Array, Dynamic Array™ and Digital Array™ IFCs with the specially designed, scalable and intuitive FC1™ cycler.

IFC Controller

IFC Controller Benefit from fewer steps and automated setup of our Access Array IFCs with Fluidigm’s fully integrated IFC Controller AX.
  1. FC1 Cycler
  2. IFC Controller

Software

Access Array™ Firmware and Software Update

Firmware and software update v2.8

Access Array™ FC1™ Cycler Software

FC1 Cycler software update to enable running the 48.48 Targeted DNA Sequencing Library Preparation system protocol on the Access Array™ system. Version 1.6

  1. Access Array™ Firmware and Software Update
  2. Access Array™ FC1™ Cycler Software

Specifications

IFC Controller AX

Dimensions
Height 33 cm (13 in)
Width 24.1 cm (9.5 in)
Depth 48.3 cm (19 in)
Weight 27 kg (59.4 lb)
Environment
Room temperature 20–30 ºC
Relative humidity 30–80%, noncondensing
Altitude Up to 2,000 m
Electrical
Installation Category II
Input connector IEC 320-C13
Service 15 A
Input power 500 W maximum
Input voltage 100–240 VAC
Line frequency 50–60 Hz

FC1 Cycler

Dimensions
Height 20 cm (8 in)
Width 23 cm (9 in) 
Depth 48 cm (19 in)
Weight 11 kg (25 lb)
Environment
Room temperature 15–30 ºC
Relative humidity 30–70% noncondensing
Altitude Up to 2,000 m
Electrical
Installation Category II
Input connector IEC 320-C13
Service 15 A
Input power 200 W maximum
Input voltage 100–240 VAC
Line frequency 50–60 Hz