No more compromising for quality. With the Biomark™ HD system, you get reliable production-scale throughput and exquisite single-cell sensitivity, with a wide variety of sample types and chemistry choices to accommodate the most genomic applications. Now you can run entire projects from research to validation to routine screening on a single system with the flexibility you want and the consistency you need. Anything else we can help with?
Design experiments to match your needs across the widest range of genomic applications.
Detect unique molecular signatures in individual cells and rare cell populations.
Accelerate discovery with a higher throughput and automated workflow.
Conserve precious samples—and funding—with lower reagent use and nanoliter reactions.
Fluidigm’s revolutionary integrated fluidic circuits (IFCs) empower life science research by automating PCR reactions in nanoliter volumes. This means using less sample and reagent, and a single microfluidic device, to achieve the high-quality, consistent results your work depends on. The Biomark HD system runs IFCs in either real-time or end-point read modes, bringing flexible, efficient and economical PCR solutions to a range of applications.
Prime the Dynamic Array IFC to prepare it for sample transfer.
Pipet samples and primer-probe sets into the designated inlets on the IFC.
Load the IFC on the controller, which prepares the reactions.
Place the IFC into Biomark for thermal cycling and end-point or real-time fluorescence detection.
View and analyze color-coded expression heat maps, allele maps and cluster plots for single or multiple runs with our analysis software.
IFCs, Assays and Reagents
Dynamic Array IFCs
Experience a new level of efficiency and throughput for genotyping applications with Fluidigm’s Dynamic Array IFCs.
Digital Array IFCs
Ensure a precise determination of copy number variation with this innovative IFC designed specifically for digital PCR experiments.
D3 Assay Design
Simplify your assay design with our D3™ tool, which enables you to easily define parameters for customized gene expression, genotyping and targeted DNA sequencing.
Delta Gene Assays
Specify your genes of interest and target species to order our custom high-quality, economical and MIQE-compliant Delta Gene™ assays.
SNP Type Assays
Leverage a flexible, economical solution for single nucleotide polymorphism (SNP) genotyping with Fluidigm's SNP Type™ assays.
Singular Analysis Toolset
An innovative, open-source solution for analyzing and visualizing data, the Singular™ Analysis Toolset offers powerful methods for identifying gene expression and mutation patterns at the single-cell level. Designed for use with Biomark™ and C1™ systems.
Real-Time PCR Analysis
Quick and efficient Real-Time PCR Analysis software helps you easily establish standard curves, calculate delta delta Ct and automatically determine the ideal threshold setting for each run. Specifically created for Biomark™ systems.
Digital PCR Analysis
Featuring tools specific to digital PCR, our innovative software offers real-time analysis, automated copy number variation, end-point imaging and the ability to quantitate up to four targets per sample using multiple reporter dyes. Designed for use with Biomark™ and EP1™ systems.
Dimensions as Shipped
|Height||58 in (147 cm)|
|Width||40 in (102 cm)|
|Depth||40 in (102 cm)|
|Weight||459 lb (208 kg)|
|Height||39.4 in (100 cm)|
|Width||29.5 in (75 cm)|
|Depth||29.5 in (75 cm)|
|Weight||308 lb (140 kg)|
|Height||13 in (33 cm)|
|Width||9.5 in (24 cm)|
|Depth||19 in (48 cm)|
|IFC||Throughput (reactions per run)|
|48.48 Dynamic Array™||2,304|
|96.96 Dynamic Array||9,216|
|12.765 Digital Array™||9,180|
|48.770 Digital Array||36,960|
|192.24 Dynamic Array||4,608|
|Flex Six™ Dynamic Array||144 x 6 separate partitions|
|LED Excitation Sources (center, in nm)||
475-41, 530-16, 575-31
|Emission Filters (center, in nm)||
525-25, 570-30, 630-30
|Detection||CCD camera; 9MPixel, 12 µm; 12 µm pixel size|
Peltier-based, ranging from 4 ºC to 99 ºC
Fluidigm Real-Time PCR Analysis Software
|Computer and Accessories||Windows XP PRO, having at least 2 GB memory, 300 GB hard drive, 4 USB ports, LCD flat screen monitor, keyboard and mouse|
|Data Storage Options||1 GB/sec Ethernet connection; 40 GB hard disk space; USB port for memory stick|