The Revolution at Hand
The world’s first automated solution for single-cell genomics research continues to break new ground, now offering more capabilities than ever before. C1 lets you prepare single-cell templates for mRNA sequencing, DNA sequencing, epigenetics or miRNA expression. Now you can conduct large-scale whole transcriptome studies to survey heterogeneity and discover novel cell populations. And with our new single-cell mRNA Seq HT workflow, you can reliably isolate and process up to 800 individual cells per run. Though we know you'll do much more than that.
Learn more about doing bigger, bolder studies with single-cell mRNA Seq HT.
Discover differences in heterogeneous populations with single-cell accuracy
Easily isolate and process individual cells with a streamlined workflow and intuitive interface
Minimize hands-on time with an automated cell-to-data workflow
Explore everything from mRNA to epigenetics, or build your own applications with C1 Open App
The innovative C1 system carefully isolates single cells into individual reaction chambers in Fluidigm's exclusive integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers or reporter genes. After staining, cells are automatically lysed and template is quickly prepared for qPCR or sequencing analysis—all in as little as four hours.
Prime the C1 IFC in preparation for sample transfer.
Insert the IFC into C1 to automatically isolate up to 800 individual cells to separate reaction chambers.
Label each cell and microscopically image the IFC to confirm cell count and viability.
Reinsert the IFC into C1 to lyse each cell for amplification.
Magnify each cell’s genomic content for sequencing or qPCR detection.
Collect product from the C1 outlet.
IFCs, Assays and Reagents
C1 mRNA Seq IFC
Begin exploring cellular heterogeneity with the C1 mRNA Seq IFC. Designed for absolute quantification or full transcript sequencing, our 96-cell IFC gives you access to the whole transcriptome and captures cells 5–25 µm in diameter.
C1 mRNA Seq HT IFC
Go bigger and bolder—discover novel cell populations or rare cell types with this innovative IFC. Capture and process up to 800 cells per run with on-IFC sample multiplexing and lower library prep costs. Designed to capture cells 10–17 µm in diameter.
C1 DNA Seq IFC
Understand the clonal context of somatic mutations to reveal the true heterogeneity within your sample. One integrated workflow lets you isolate and process 96 cells to explore cell lineage at the whole genome, whole exome or targeted loci level. Designed to capture cells 5–25 µm in diameter.
C1 Preamp IFC
The C1 Preamp IFC enables targeted gene expression to verify gene signatures defining a cell population or delve into the miRNA regulating gene expression. Designed to capture cells 5–25 µm in diameter and for use with Biomark systems.
C1 Open App IFC
C1 Reagent Kit
Jump-start your single-cell studies with our ready-to-use reagent kits for mRNA sequencing, DNA sequencing, preamplification and Open App use. Each kit contains the components needed for cell loading, lysis and harvesting.
Singular Analysis Toolset
An innovative, open-source solution for analyzing and visualizing data, the Singular™ Analysis Toolset offers powerful methods for identifying gene expression and mutation patterns at the single-cell level. Designed for use with Biomark™ and C1™ systems.
C1 System Software v2.0
C1 mRNA Seq HT on Script Hub
Script Hub™ is your central source for C1™ methods and applications. Get access to new single-cell mRNA Seq HT scripts and other applications developed by Fluidigm and the C1 community, including epigenetics, mRNA sequencing, DNA sequencing, cell staining and more.
|Clearance||6 in (15 cm) from the rear|
|Temperature||59–82 °F (15–28 °C) stable|
|Altitude||<8,202 ft (2,500 m)|
Refer to the C1 System Site Requirements (PN 100-5201) for more information on operating requirements.
|Height||30 in (76 cm)|
|Width||28 in (71 cm)|
|Depth||39 in (99 cm)|
|Weight||159 lb (72 kg)|
|Height||19 in (48 cm)|
|Width||16 in (41 cm)|
|Depth||26 (66 cm)|
|Weight||103 lb (46.7 kg)|
|Thermal control||Peltier-based, 4–99 °C|
|Max heating rate||>4 °C/sec|
|Max cooling rate||>3 °C/sec|
|IFC compatibility||C1 IFCs|
|Current||9 A (max)|
|Main supply fluctuation||Not to exceed ±10% of the nominal supply voltage|