The Ultimate Endpoint
The most efficient system for high-sample-throughput SNP genotyping and other endpoint PCR applications also allows for extremely low running costs. Plus, the EP1™ system provides the easiest workflow for low- to mid-multiplex SNP genotyping. Your work demands the best. And we're happy to oblige.
Dramatically lower costs with high-sample-format IFCs.
Obtain results in hours with only minutes of hands-on time.
Achieve higher throughput with a scalable workflow.
Enjoy high-confidence calls based on superior data quality.
Fluidigm's exclusive integrated fluidic circuits (IFCs) empower life science research by automating molecular biology in nanoliter volumes. This means using less sample and reagent, and a single microfluidic device, to achieve the high-quality, consistent results your work depends on. Using an endpoint reader, thermal cycler and IFC controllers customized for your throughput requirements, the EP1 system offers the most efficient and economical solution for low- to mid-multiplex SNP genotyping and digital PCR.
Prime the Dynamic Array IFC to prepare it for sample transfer.
Pipet samples and primer-probe sets into the designated inlets on the IFC.
Set the IFC on the controller to automatically set up reaction chambers.
Place the IFC on the FC1™ and start the PCR protocol.
Place the IFC on the EP1 Reader for fluorescence detection.
View and analyze color-coded allele maps and cluster diagrams for a single or multiple runs with our analysis software.
Biomark & EP1 Support
Explore tutorials and publications, browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
IFCs, Assays and Reagents
Digital Array IFCs
Ensure a precise determination of copy number variation with this innovative IFC designed specifically for digital PCR experiments.
Biomark & EP1 Software
The Biomark/EP1 software package includes:
Data Collection Software v4.2.2
SNP Genotyping Analysis v4.3.2
SNP Genotyping software analyzes genotyping data acquired from Biomark™ or EP1 systems. Advanced features include a cluster-calling algorithm, multiple IFC scoring and automatic confidence score ratings.
Real-Time PCR Analysis v4.1.3
Real-Time PCR Analysis software helps you quickly and easily create standard curves, calculate DDCt and automatically determine the ideal threshold setting for each run. Specifically created for Biomark™ systems.
Digital PCR Analysis v4.1.2
Our innovative Digital PCR Analysis software offers real-time and endpoint dPCR analysis,
automated copy number variation, and the ability to quantitate up to four targets per sample.
Designed for use with Biomark™ and EP1™ systems.
Melt Curve Analysis v4.1.3
|Sensitivity||Single copies present in reaction chambers are routinely detected (using TaqMan® chemistry).|
|Excitation filters (center, in nm)||485, 530, 580 (two empty positions)|
|Emission filters (center, in nm)||525, 570, 630 (two empty positions)|
|Illumination||175-watt xenon arc lamp|
|Thermal control||Peltier-based, ranging from 4 ºC to 99 ºC|
|Max heating ramp rate: >5 ºC/sec|
|Max cooling ramp rate: >5 ºC/sec|
|Software||Real-time PCR Analysis software, Genotyping Analysis software, Digital PCR Analysis software, Data Collection software|
|Computer and accessories||Internal CPU; LCD flat screen monitor; wireless keyboard and mouse|
|Data storage options||1 GB/sec Ethernet connection; 40+ GB hard disk space; USB port for memory stick|
|Installation||Installation kit; onsite installation and training|