EP1

Efficient, economical endpoint PCR results in hours with just minutes of hands-on time

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The Ultimate Endpoint

The most efficient system for high-sample-throughput SNP genotyping and other endpoint PCR applications also allows for extremely low running costs. Plus, the EP1™ system provides the easiest workflow for low- to mid-multiplex SNP genotyping. Your work demands the best. And we're happy to oblige.

Economical

Dramatically lower costs with high-sample-format IFCs.

Fast

Obtain results in hours with only minutes of hands-on time.

Efficient

Achieve higher throughput with a scalable workflow.

Quality

Enjoy high-confidence calls based on superior data quality.

Technology

Fluidigm's exclusive integrated fluidic circuits (IFCs) empower life science research by automating molecular biology in nanoliter volumes. This means using less sample and reagent, and a single microfluidic device, to achieve the high-quality, consistent results your work depends on. Using an endpoint reader, thermal cycler and IFC controllers customized for your throughput requirements, the EP1 system offers the most efficient and economical solution for low- to mid-multiplex SNP genotyping and digital PCR.

Workflow

  1. Prime
  2. Transfer
  3. Load
  4. Cycle
  5. Read
  6. Analyze

Prime

Prime the Dynamic Array IFC to prepare it for sample transfer.

Transfer

Pipet samples and primer-probe sets into the designated inlets on the IFC.

Load

Set the IFC on the controller to automatically set up reaction chambers.

Cycle

Place the IFC on the FC1™ and start the PCR protocol.

Read

Place the IFC on the EP1 Reader for fluorescence detection.

Analyze

View and analyze color-coded allele maps and cluster diagrams for a single or multiple runs with our analysis software.

Support

Biomark & EP1 Support

Explore tutorials and publications, browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here. 

components

IFCs, Assays and Reagents

Digital Array IFCs

Ensure a precise determination of copy number variation with this innovative IFC designed specifically for digital PCR experiments.

Dynamic Array IFCs

Experience a new level of efficiency and throughput for gene expression and genotyping applications with the Fluidigm Dynamic Array™ IFCs used together with the Juno and Biomark HD systems.

  1. Digital Array IFCs
  2. Dynamic Array IFCs

Related Systems

IFC Controllers

IFC Controllers Benefit from fewer steps and automated setup of our Dynamic Array™ and Digital Array™ IFCs with Fluidigm’s highly efficient IFC controllers.

FC1 Cycler

FC1 Cycler Optimize thermal cycling of your Access Array, Dynamic Array™ and Digital Array™ IFCs with the specially designed, scalable and intuitive FC1™ cycler.
  1. IFC Controllers
  2. FC1 Cycler

Software

Biomark & EP1 Software

The Biomark/EP1 software package includes:

Data Collection Software v4.2.2

fluidigm

SNP Genotyping Analysis v4.3.2
SNP Genotyping software analyzes genotyping data acquired from Biomark™ or EP1™systems. Advanced features include a cluster-calling algorithm, multiple IFC scoring and automatic confidence score ratings.

fluidigm

Real-Time PCR Analysis v4.3.1
Real-Time PCR Analysis software helps you quickly and easily create standard curves, calculate DDCt and automatically determine the ideal threshold setting for each run. Specifically created for Biomark  systems.

fluidigm

Digital PCR Analysis v4.1.2
Our innovative Digital PCR Analysis software offers real-time and endpoint dPCR analysis,
automated copy number variation, and the ability to quantitate up to four targets per sample.
Designed for use with Biomark and EP1 systems.

fluidigm

Melt Curve Analysis v4.1.3

  1. Biomark & EP1 Software

Specifications

Performance

Sensitivity Single copies present in reaction chambers are routinely detected (using TaqMan® chemistry).

Components

Excitation filters (center, in nm) 485, 530, 580 (two empty positions)
Emission filters (center, in nm) 525, 570, 630 (two empty positions)
Detection CCD camera
Illumination 175-watt xenon arc lamp
Thermal control Peltier-based, ranging from 4 ºC to 99 ºC
Max heating ramp rate: >5 ºC/sec
Max cooling ramp rate: >5 ºC/sec
Software Real-time PCR Analysis software, Genotyping Analysis software, Digital PCR Analysis software, Data Collection software
Computer and accessories Internal CPU; LCD flat screen monitor; wireless keyboard and mouse
Data storage options 1 GB/sec Ethernet connection; 40+ GB hard disk space; USB port for memory stick

Startup Support

Installation Installation kit; onsite installation and training