Juno

SNP genotyping of challenging samples in three hours with one hands-on step

Product

Hands-Free Biology

How would you like to genotype from as little as 5.5 ng total input DNA and get 9,216 data points in under three hours with a single touch of a button? Introducing Juno™, a first-of-its-kind revolution that combines preamplification and SNP genotyping into one automated process. Now researchers in genetic health, agrigenomics, sample identification and more can accurately genotype extremely small and challenging samples with minimal hands-on time. All in a sleek, beautifully designed machine that looks like a work of art. It may not be polite to stare, but no worries. We're doing it too.

Minimal

Get precise data from a starting concentration of as little as 2.5 ng/µL, 5.5 ng total DNA

Easy

Transfer samples and assays, press Start and have complete results within three hours

Flexible

Take advantage of a wide selection of sample and assay configurations without added costs

Intuitive

Enjoy an advanced user interface and modern design that enhances your experience

Applications

Technology

Juno integrates IFC control and thermal cycling to enable hands-free genotyping from low sample amounts and eliminate the hassle of harvesting and transferring preamplified DNA. An all-new, specially designed integrated fluidic circuit (IFC) combines the preamplification and SNP genotyping processes using multiple layers and advanced valve controls. This lets you set up one IFC to perform multiple steps, leaving you free to focus on the science.

Workflow

  1. Prime
  2. Transfer
  3. Load
  4. Run
  5. Analyze

Prime

Prime the Dynamic Array IFC to prepare it for sample transfer.

Transfer

Pipette samples and primer-probe sets into the designated inlets on the IFC.

Load

Load the IFC on the controller, which prepares the reactions.

Run

Place the IFC into Biomark for thermal cycling and end-point or real-time fluorescence detection.

Analyze

View and analyze color-coded expression heat maps, allele maps and cluster plots for single or multiple runs with our analysis software.

  1. Prime
  2. Transfer
  3. Load
  4. Cycle
  5. Read
  6. Analyze

Prime

Prime the Dynamic Array IFC to prepare it for sample transfer.

Transfer

Pipette samples and primer-probe sets into the designated inlets on the IFC.

Load

Set the IFC on the controller to automatically set up reaction chambers.

Cycle

Place the IFC on the FC1™ and start the PCR protocol.

Read

Place the IFC on the EP1 Reader for fluorescence detection.

Analyze

View and analyze color-coded allele maps and cluster diagrams for a single or multiple runs with our analysis software.

  1. Transfer
  2. Run
  3. Read

Transfer

Pipette samples and assays into the designated inlets on the IFC.

Run

Place the IFC in Juno and press Start.

 

Read

View the IFC results on your Biomark™ HD system.

 

components

IFCs, Assays and Reagents

Dynamic Array IFCs

Experience a new level of efficiency and throughput for genotyping applications with Fluidigm’s Dynamic Array IFCs.

Digital Array IFCs

Ensure a precise determination of copy number variation with this innovative IFC designed specifically for digital PCR experiments.

Juno 96.96 Genotyping IFC

Transform your workflow with this innovative IFC, which leverages multiple layers and advanced valve controls to achieve preamplification and SNP genotyping in one step.

 

SNP Type Assays

Leverage a flexible, economical solution for single nucleotide polymorphism (SNP) genotyping with Fluidigm's SNP Type™ assays.

D3 Assay Design

Simplify your assay design with our D3™ tool, which enables you to easily define parameters for customized gene expression, genotyping and targeted DNA sequencing.

Delta Gene Assays

Specify your genes of interest and target species to order our custom high-quality, economical and MIQE-compliant Delta Gene™ assays.

SNP Trace™ Panel

Ensure accurate sample identification with an authoritative panel of 96 SNPs that determine specific attributes around gender, ancestry or polymorphism across populations.

  1. Dynamic Array IFCs
  2. Digital Array IFCs
  3. Juno 96.96 Genotyping IFC
  4. SNP Type Assays
  5. D3 Assay Design
  6. Delta Gene Assays
  7. SNP Trace™ Panel

Related Systems

IFC Controllers

IFC Controllers Benefit from fewer steps and automated setup of our Dynamic Array and Digital Array IFCs with Fluidigm’s highly efficient IFC Controllers.

Biomark HD

Biomark HD Analyze single-cell gene expression with real-time PCR, industry-leading flexibility and consistently high-quality results on the Biomark™ HD system.

FC1 Cycler

FC1 Cycler Optimize thermal cycling of your Access Array, Dynamic Array and Digital Array IFCs with the specially designed, scalable and intuitive FC1 cycler.
  1. IFC Controllers
  2. Biomark HD
  3. FC1 Cycler

Software

SNP Genotyping Analysis

Highly accurate genotyping analysis software addresses genotyping's specific needs with an advanced automatic cluster-calling algorithm, multiple chip scoring and automatic confidence score. Created for use with Biomark, EP1™ and Juno™ systems.

Digital PCR Analysis

Featuring tools specific to digital PCR, our innovative software offers real-time analysis, automated copy number variation, end-point imaging and the ability to quantitate up to four targets per sample using multiple reporter dyes. Designed for use with Biomark and EP1 systems. 

Singular Analysis Toolset

An innovative, open-source solution for analyzing and visualizing data, the Singular™ Analysis Toolset offers powerful methods for identifying gene expression and mutation patterns at the single-cell level. Designed for use with Biomark™ and C1™ systems.

Real-Time PCR Analysis

Quick and efficient Real-Time PCR Analysis software helps you easily establish standard curves, calculate delta delta Ct and automatically determine the ideal threshold setting for each run. Specifically created for Biomark systems. 

  1. SNP Genotyping Analysis
  2. Digital PCR Analysis
  3. Singular Analysis Toolset
  4. Real-Time PCR Analysis

Specifications

Dimensions as Shipped

Height 58 in (147 cm)
Width 40 in (102 cm)
Depth 40 in (102 cm)
Weight 459 lb (208 kg)

Uncrated

Height 39.4 in (100 cm)
Width 29.5 in (75 cm)
Depth 29.5 in (75 cm)
Weight 308 lb (140 kg)

IFC Controller

Height 13 in (33 cm)
Width 9.5 in (24 cm)
Depth 19 in (48 cm)

Performance

IFC Throughput (reactions per run)
48.48 Dynamic Array 2,304
96.96 Dynamic Array 9,216
12.765 Digital Array 9,180
48.770 Digital Array 36,960
192.24 Dynamic Array 4,608
Flex Six™ Dynamic Array 144 x 6 separate partitions

Components

LED Excitation Sources (center, in nm)

475-41, 530-16, 575-31
Optional: 632-27

Emission Filters (center, in nm)

525-25, 570-30, 630-30
Optional: 700-30

Detection CCD camera; 9MPixel, 12 µm; 12 µm pixel size
Thermal Control

Peltier-based, ranging from 4ºC - 99ºC
Max heating rate: > 5ºC/sec
Max cooling rate: > 5ºC/sec

Software

Fluidigm Real-Time PCR Analysis Software
Fluidigm SNP Genotyping Analysis Software
Fluidigm Digital PCR Analysis Software
Fluidigm Data Collection Software

Computer and Accessories Windows XP PRO, having at least 2 GB memory, 300 GB hard drive, 4 USB ports, LCD flat screen monitor, keyboard and mouse
Data Storage Options 1 GB/sec Ethernet connection; 40 GB hard disk space; USB port for memory stick

Performance

Sensitivity Single copies present in reaction chambers are routinely detected (using TaqMan® chemistry)

Components

Excitation Filters(center, in nm) 485, 530, 580 (two empty positions)
Emission Filters(center, in nm) 525, 570, 630 (two empty positions)
Detection CCD camera
Illumination 175-watt Xenon arc lamp
Thermal Control Peltier-based, ranging from 4ºC - 99ºC
Max Heating ramp rate: >5ºC/sec
Max Cooling ramp rate: >5ºC/sec
Software Real-time PCR Analysis SoftwareGenotyping Analysis SoftwareDigital PCR Analysis SoftwareData Collection Software
Computer and Accessories Internal CPU; LCD flat screen monitor; wireless keyboard and mouse
Data Storage Options 1 GB/sec Ethernet connection; 40+ GB hard disk space; USB port for memory stick

Startup Support

Installation Installation kit; On-site installation and training

Dimensions

Height 19.7in (50 cm)
Width 17.3 in (43.9 cm)
Depth 27.5 in (69.8 cm)
Weight 137 lb (62 kg)

Thermal Control

Peltier-based 4–99 ºC
Max heating rate >5 ºC/sec
Max cooling rate >5 ºC/sec

IFC Compatibility

Dynamic Array™ IFCs 48.48, 96.96, 192.24, Flex Six
Digital Array™ IFCs 12.765, 48.770, qdPCR 37K

Data Storage

Ethernet connection 1GB/sec
Hard drive 40GB
Port USB port
Computer Internal CPU
touchscreen interface