Mass Cytometry

Driving groundbreaking discoveries one cell at a time, millions of times

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Mass cytometry by time-of-flight, used in CyTOF® systems, is the world’s most advanced single-cell proteomics technology. It has opened new doors to discovery by empowering researchers to interrogate more than 40 markers simultaneously on millions of individual cells, to unveil new cell types, functions and biomarkers in immunology, cancer, stem cells and more. Fluidigm Maxpar® reagents and protocols and our Panel Designer tool provide all you need for groundbreaking and cost-effective experiments that reveal systems-level biology at single-cell resolution.

What's New

Recap | 6th Annual Mass Cytometry Summit

On June 9, 2017, more than 200 researchers from around the world gathered in Boston for the 6th year in a row to share their work, discuss advances and develop new collaborations. Many of the talks are now available on demand.

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Helios Mass Cytometry News

Maxpar Human T Cell Immuno-Oncology EX Panel Kit

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Mass Cytometry Webinars

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Methods

Sample Applications

Current CyTOF systems, including the latest mass cytometer, Helios™, provide high-parametric data on individual cells prepared in liquid suspension. Standard protocols for preparing single-cell suspensions from tissue culture and solid and liquid tissues as well as tumors can be used for CyTOF sample prep. Sample size can vary from thousands to millions of cells.

Metal-Tagged Reagents

Cellular targets in liquid suspension can be labeled with more than 40 metal-tagged reagents, including antibodies, nucleic acid intercalators and analogs, as well as other biochemical ligands. Each reagent is detected and quantified with cytometry by time-of-flight mass spectrometry in the CyTOF system. The high purity and choice of metal isotopes ensure minimal background noise from signal overlap or endogenous cellular components. Our catalog is stocked with over 600 antibodies detecting more than 400 unique human or mouse targets. If a desired antibody is not yet present in our catalog, you can make it yourself using our metal-labeling kits or custom order biologically validated material using our custom conjugation services.

Cell Profiling

Targets can be anywhere in the cell, including the surface, cytoplasm, nucleus or internal organelles. Complete profiling allows CyTOF researchers to investigate a range of applications, including phenotyping, functional activity, apoptosis and cell cycle analysis. Phosphoprotein detection can be used to map the signaling profile of samples, and optimized protocols and detailed technical assistance are available for any combination of targets.

Barcoding for enhanced efficiency and quality

Barcode reagents allow unique metal-ion tagging of up to 20 individual liquid suspension samples, offering increased throughput, improved protocol consistency and enhanced data quality. Barcoding also enables efficient use of small precious samples through multiplexing and may provide cost savings through reduced consumption of reagents. Barcoding is a powerful enabling component for any experiment involving multiple samples, time courses and dose-response assays.

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