172 results found in Ag-Genomics
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User Guide
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article
Amplify Your Productivity
With more than 1,000 publications and counting, Biomark is a rising star in PCR …
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Publications
Foliar treatments with Gaultheria procumbens essential oil induce defense responses and resistance against a fungal pathogen in Arabidopsis
Essential oil from Gaultheria procumbens is mainly composed of methylsalicylate (MeSA) (>96%), a compound which can be metabolized in plant tissues to salicylic acid, a phytohormone inducing plant immunity against microbial pathogens. …
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Publications
Age-dependent expression of osteochondrosis-related genes in equine leukocytes
Osteochondrosis (OC) is a developmental disease in horses which has a significant impact on the horse's welfare and performance. …
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Publications
The 25 Best Inventions of 2015 - Time Magazine
In its annual lineup of innovative products that make the world smarter, simpler, more sustainable and even more enjoyable, Time magazine chose Juno™ as one of the 25 best of the year. …
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Publications
Accurate Determination of Genetic Identity for a Single Cacao Bean, Using Molecular Markers with a Nanofluidic System, Ensures Cocoa Authentication
Cacao (Theobroma cacao L.), the source of cocoa, is an economically important tropical crop. …
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article
Building a Better Tomato
Molecular marker breeding is leading to seed innovations at Bayer CropScience …
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article
Prime Time for Juno
Time magazine ranks Juno among ‘25 Best Inventions of 2015’ …
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article
A Meaningful Contribution
Founder Gajus Worthington on advancing single-cell biology …
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system
C1 Open App
Breakthrough single-cell applications, developed by and for the C1 community …
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article
Eureka! Easy Genotyping for Tough Samples
The why of Juno …
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media
Creative Science: The Next Generation
See how today’s young scientists are making a dramatic impact in STEM fields …
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faq
What genotyping chemistries can be used on the Fluidigm platform?
Fluidigm supports the use of its SNP Type assays and the TaqMan® SNP Genotyping Assays from Thermo Fisher Scientific. …
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faq
How do the SNP Type and TaqMan chemistries differ?
Both chemistries are robust methods for SNP genotyping, and both are compatible with specific target amplification protocols. …
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faq
What Fluidigm instruments are required for SNP genotyping?
An IFC Controller, thermal cycler and fluorescence reader are required for Fluidigm SNP genotyping. …
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faq
Can I change the thermal cycling conditions for my genotyping reactions?
While it is possible to modify existing thermal protocols or create custom thermal protocols, Fluidigm does not support protocol variations other than those listed in the SNP Genotyping User Guide (PN 68000098), and any deviations are at the risk of the user. …
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faq
Can I use TaqMan Assays if the stock concentration is only 20X?
Yes. When preparing assays for use in Dynamic Array IFCs, first combine equal volumes of 20X SNP Genotyping Assay mix and 2X Assay Loading Reagent, then add the recommended volume of 50X ROX Reference Dye. …
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faq
What are SNP Type Assays?
SNP Type Assays are allele-specific PCR assays made by Fluidigm that use three primers and two universal probes to distinguish between two alleles. …
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faq
What fluorophores are used for SNP Type Genotyping? How do I know which fluorophore represents which allele?
SNP Type Genotyping Assay probes are labeled with the FAM and HEX fluorophores. …
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faq
Why can't I run real-time PCR with my SNP Type Assays?
SNP Type Genotyping reactions must be imaged at room temperature (25 °C) so that any unincorporated universal probes will be hybridized to their respective quencher oligos. …