171 results found in Ag-Genomics
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faq
What is the process for redesigning assays?
The primer pooling algorithm generates 20 primer pools that are designed for minimal interactions among primers and minimal nonspecific amplification. …
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faq
Do I need to update my software on Juno?
Yes, new software is required and available for download. …
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faq
Can I use my existing Access Array kit and assays on Juno with the Juno LP—192.24 IFC?
No, the LP—192.24 IFC was designed to work specifically with the Targeted DNA Seq Library Reagent Kit on Juno™. …
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faq
Can I use automation with the Targeted DNA Seq Library Reagent Kit?
Contact Fluidigm support at tech.support@fluidigm.com or fluidigm.com/contacts for guidance. …
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faq
What information is provided in the Juno Targeted DNA Sequencing Library Prep Customer Informatics Packet?
A Microsoft® Excel® file that contains the primer sequences, genomic coordinates, GC content, multiplexed primer stock map of primer position in plates, and amplicon sequence and length. …
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faq
What species and targets do you design Targeted DNA Sequencing Library Prep assay primers to?
Targeted DNA Seq Library assays are designed to human species for targets with an associated RefSeq ID, gene name, target sequence or genome coordinate. …
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faq
What methods are supported by the Juno instrument?
Juno™ is preprogrammed to perform multiple methods with push-button ease. Juno methods include targeted DNA sequencing library preparation, IFC control and thermal cycling for SNP genotyping upstream of Biomark™ HD or EP1™ systems, and IFC control for gene expression, copy number analysis or digital PCR upstream of Biomark HD. …
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faq
What is the minimum DNA input for targeted sequencing?
100 ng input per sample for human genomic DNA or for DNA of equivalent genome size. For larger genomes, contact Fluidigm support (fluidigm.com/contacts) for guidance on input requirements. …
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faq
Do I need to upgrade my Juno system?
If your system accessories do not contain the Barrier Tape Applicator, TX Interface Plate and 7 Drawer Interface Plate holder, you will need to order the Juno™ LP Upgrade Kit (101-2026). …
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article
Amplify Your Productivity
With more than 1,000 publications and counting, Biomark is a rising star in PCR …
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Publications
Foliar treatments with Gaultheria procumbens essential oil induce defense responses and resistance against a fungal pathogen in Arabidopsis
Essential oil from Gaultheria procumbens is mainly composed of methylsalicylate (MeSA) (>96%), a compound which can be metabolized in plant tissues to salicylic acid, a phytohormone inducing plant immunity against microbial pathogens. …
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Publications
Age-dependent expression of osteochondrosis-related genes in equine leukocytes
Osteochondrosis (OC) is a developmental disease in horses which has a significant impact on the horse's welfare and performance. …
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Publications
The 25 Best Inventions of 2015 - Time Magazine
In its annual lineup of innovative products that make the world smarter, simpler, more sustainable and even more enjoyable, Time magazine chose Juno™ as one of the 25 best of the year. …
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Publications
Accurate Determination of Genetic Identity for a Single Cacao Bean, Using Molecular Markers with a Nanofluidic System, Ensures Cocoa Authentication
Cacao (Theobroma cacao L.), the source of cocoa, is an economically important tropical crop. …
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article
A Meaningful Contribution
Founder Gajus Worthington on advancing single-cell biology …
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system
C1 Open App
Breakthrough single-cell applications, developed by and for the C1 community …
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media
Creative Science: The Next Generation
See how today’s young scientists are making a dramatic impact in STEM fields …
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faq
What genotyping chemistries can be used on the Fluidigm platform?
Fluidigm supports the use of its SNP Type assays and the TaqMan® SNP Genotyping Assays from Thermo Fisher Scientific. …
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faq
How do the SNP Type and TaqMan chemistries differ?
Both chemistries are robust methods for SNP genotyping, and both are compatible with specific target amplification protocols. …
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faq
What Fluidigm instruments are required for SNP genotyping?
An IFC Controller, thermal cycler and fluorescence reader are required for Fluidigm SNP genotyping. …