167 results found for "TaqMan"
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faq
What master mixes can I use with TaqMan gene expression assays on Fluidigm IFCs?
For fast thermal cycling conditions, the following master mixes can be used: …
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faq
Is the 24.192 GE IFC compatible with Fast TaqMan chemistry?
The 24.192 GE IFC can be used with Fast TaqMan® chemistry. …
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faq
What is the difference between EvaGreen and TaqMan?
TaqMan® assays (Thermo Fisher Scientific) are primer and probe sets that are designed to individual genes and provide very specific detection of gene expression. …
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faq
How do the SNP Type and TaqMan chemistries differ?
Both chemistries are robust methods for SNP genotyping, and both are compatible with specific target amplification protocols. …
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faq
Can I use TaqMan Assays if the stock concentration is only 20X?
Yes. When preparing assays for use in Dynamic Array IFCs, first combine equal volumes of 20X SNP Genotyping Assay mix and 2X Assay Loading Reagent, then add the recommended volume of 50X ROX Reference Dye. …
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technical note
Single-Cell Gene Expression Profiling using TaqMan Gene Expression Assays with the C1 System
Using TaqMan Gene Expression Assays for Specific Target Amplification (STA) with the C1 Single-Cell Auto Prep System.
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faq
What chemistry is used for the Advanta IO Gene Expression Assay?
Thermo Fisher Scientific Applied Biosystems® TaqMan® probes and primers. …
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faq
Is it possible to add assays to the Advanta IO Gene Expression Assay?
Yes, 17 open assay inlets on Panel B are available for additional TaqMan® assays. Assays (at 20X concentration) can be sourced directly from Thermo Fisher Scientific. …
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faq
What gene expression chemistries are compatible with the 24.192 GE IFC?
The 24.192 GE IFC can be used with TaqMan® chemistry. Contact tech support at techsupport@fluidigm.com if you would like to use TaqMan chemistry in fast-cycling mode. …
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Quick Reference
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Quick Reference
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Protocol
C1 System for TaqMan Assays Protocol
Using C1 to Capture Cells from Cell Culture and Perform Preamplification Using TaqMan Assays
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faq
Which genes should I use as reference with the Advanta IO Gene Expression Assay to normalize expression?
Five reference genes included in both Panel A and Panel B may be used as reference genes. The expression level of genes selected as a reference should be independent of the conditions under investigation. …
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Protocol
C1 System for microRNA (TaqMan) Protocol
Using C1 to Capture Cells from Cell Culture and Perform Preamplification of MicroRNA Species Using TaqMan Assays
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faq
What PCR master mixes are supported for genotyping?
Fluidigm currently supports the use of the following master mixes: …
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Quick Reference
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Quick Reference