151 results found for "ffpe"
-
faq
How much of my FFPE sample should I load on the Access Array?
For a successful Access Array run with FFPE sample, we recommend that users check the quality of the samples and complete a 180 bp TaqMan® copy number assessment. …
-
faq
Can I use the kit to extract DNA from FFPE samples?
No, you cannot use this kit to extract DNA. …
-
faq
What types of samples could be used with the Advanta FFPE RNA Extraction Kit?
Extraction of RNA could be done using FFPE samples in forms of slides, curls and sections, core needle biopsies, or fine needle aspirates. …
-
faq
How many samples could be processed with the Advanta FFPE RNA Extraction Kit?
The kit contains sufficient reagents to perform 50 extractions. …
-
faq
What is the final elution volume of the Advanta FFPE RNA Extraction Kit Protocol?
The final elution volume is 20 μL. It is recommended to retrieve no more than 18 μL to avoid drawing out beads into the final RNA suspension solution. …
-
faq
What do you recommend as FFPE RNA reference material for control?
We recommend using the 5 Fusion Multiplex RNA Negative Control (Horizon Discovery, PN HD783) for a control. …
-
faq
How long can I store extracted material?
Extracted material is stable for up to 30 days when stored at –80 °C and up to 1 week at –20 °C. …
-
faq
What kind of magnetic separator do you recommend?
We recommend using the DynaMag™-2 Magnet from Thermo Fisher Scientific (PN 12321D). …
-
faq
Can I purchase ethanol from any vendor?
Yes, you can purchase ethyl alcohol from any vendor as long as it is pure ethyl alcohol, 200 proof, molecular biology grade. …
-
faq
Can I purchase hexadecane from any vendor?
Yes, you can purchase hexadecane from any vendor. Hexadecane needs to be of ≥99% purity. …
-
faq
How much tissue should I start with?
The Advanta™ FFPE RNA Extraction Kit Protocol (PN 101-6554) is applicable for extraction of RNA from FFPE tissue sections that are 5–10 µm thick with an area of 20–200 mm2, for a total volume of up to 2.0 mm3 of tissue in each reaction. …
-
faq
If my gDNA is less than the minimum required concentration for genotyping, what can I do?
If the minimum gDNA concentration cannot be met, we recommend preamplification prior to genotyping. …
-
faq
Why do I need to prepare fresh 80% ethanol?
Diluted ethanol evaporates over time, and its concentration changes. …
-
consumable
Advanta™ FFPE RNA Extraction Kit
This kit enables a rapid and a highly efficient extraction of RNA from formalin-fixed, paraffin-embedded (FFPE) tissue. The reagent kit includes sufficient reagents to extract RNA from up to 50 samples. …
Product #: 101-6773
-
faq
For which applications can I use extracted RNA?
Extracted RNA can be used for any downstream application that requires RNA sample as an input for gene expression analysis and profiling, including real-time PCR and RNA-seq. …
-
faq
Can I prepare a master mix for steps that require mixing multiple reagents?
A few steps that direct the user to pipet reagents individually into each sample tube can be modified into a master mix preparation for easier pipetting when more than a few sample tubes are processed at a time. …
-
faq
What samples can I use with the Hyperion Imaging System?
The Hyperion™ Imaging System can image biological samples including frozen or formalin-fixed, paraffin-embedded (FFPE) tissue sections or cell smears (liquid biopsies) deposited onto glass slides. …
-
faq
My extracted RNA yield is lower than expected. What could be causing that?
Lower RNA yield could be caused by (a) the loss of tissue sample during the hexadecane and/or 100% ethanol removal steps of the Advanta™ FFPE RNA Extraction Kit Protocol or (b) the type of magnetic stand used. …
-
faq
What is DV200?
DV200 represents the percentage of RNA fragments that are >200 nucleotides in size. …
-
faq
Can I still use RNA integrity number (RIN)?
The RNA integrity number (RIN) is an algorithm for assigning integrity values to RNA measurements using the 28S-to-18S-rRNA ratio. Both the 28S and 18S rRNA are fragmented in the RNA extracted from FFPE samples. Therefore, RIN may not be a relevant metric for FFPE-derived RNA samples. …