201 results found for "preamp"
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faq
Do I need preamplification for gene expression? Why?
Preamplification is recommended for all cDNA samples because of the nanoscale volume of Fluidigm IFCs. …
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faq
Do I have to dilute my reaction products 5X after preamplification?
We recommend a minimum five-fold dilution after preamplification to sufficiently dilute the preamplification reaction components. …
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faq
What is the maximum number of preamplification cycles I can do?
No maximum number of preamplification cycles has been established. …
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faq
How should I dilute preamplification products after amplification?
To help protect against degradation during storage, preamplification products should be diluted in a DNase/RNase-free, low-EDTA TE buffer (10 mM Tris, 0.1 mM EDTA; pH 8.0) after preamplification. …
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faq
How much RNA is needed for the reverse transcriptase and preamplification steps of RT-PCR?
For Fluidigm Reverse Transcription Master Mix, we recommend from 150 ng/μL down to 1 pg/μL of total RNA as starting material …
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faq
Will preamplification introduce amplification bias?
By limiting both the primer concentration and the number of PCR cycles, the preamplification protocol enables unbiased amplification of specific DNA targets. …
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product insert
C1 Reagent Kit for Preamplification Product Insert
This kit is used to facilitate the capture of cells and performing preamplification on single cells or for targeted gene expression analysis.
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data sheet
PreAmp Master Mix Data Sheet
The new PreAmp Master Mix from Fluidigm® is optimized for multiplex preamplification reactions with Delta Gene™or TaqMan® gene expression assays performed in Dynamic Array™ IFCs.
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faq
When is the use of preamplification recommended?
Inclusion of an off-IFC preamplification step is recommended when using samples with concentrations below 30 ng/μL or purity <1.5 (A160/A180). …
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faq
How many primer pairs can I include in a multiplex preamplification reaction?
The Fluidigm preamplification protocol has been validated for multiplex preamplification of up to 96 targets. …
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faq
When should I perform preamplification prior to SNP genotyping on the Fluidigm platform?
Preamplification can be used to improve data quality when there is: …
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faq
What is the minimum concentration of genomic DNA sample required for preamplification?
A minimum concentration of 10 ng/μL is recommended for human samples. …
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faq
Is preamplification required in the Advanta IO Gene Expression Assay?
Yes, preamplification is required to achieve the performance characteristics of the assay. …
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faq
Why is the Exo I treatment after preamplification needed only for EvaGreen?
EvaGreen® works by detecting all double-stranded DNA. …
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Protocol
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faq
Can I use master mixes other than the ones listed in the Fluidigm protocols for gene expression using Delta Gene assays?
Fluidigm supports only the master mixes listed in the protocols. …
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Quick Reference
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faq
Can SYBR Green be used?
No, SYBR™ Green is not compatible with Fluidigm IFCs. …
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Quick Reference
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faq
If my gDNA is less than the minimum required concentration for genotyping, what can I do?
If the minimum gDNA concentration cannot be met, we recommend preamplification prior to genotyping. …