The New Standard in Immune Profiling Has Arrived

30 markers. 1 tube. 5-minute data analysis.


Profile 37 immune cell populations from PBMC or whole blood with an optimized 30-marker panel.

Simple single-tube workflow with pushbutton reporting. Just add sample and go.

Produce consistent results lot-to-lot, run-to-run and site-to-site.


Introducing the Maxpar Direct Immune Profiling Assay
with Maxpar Pathsetter software

The Maxpar® Direct™ Immune Profiling System is the first complete sample-to-answer solution for high-dimensional immune profiling of human PBMC and whole blood.

Designed as a simple, single-tube workflow, the system brings together CyTOF® technology on the Helios™ system, a dry 30-marker antibody panel and automated Maxpar Pathsetter™ software so you can easily count 37 immune cell populations with performance you can trust.

The new standard in immune profiling

A. Dry single-tube panel design

Dry single-tube design

B. Just add sample and GO!

Maxpar Direct Immune Profiling Assay workflow

Figure 1. The new standard in immune profiling
All 30 antibodies are provided dry in a single tube for easy sample addition. Cell-ID™ Intercalator-103Rh is included in each tube to measure cell viability. B. The streamlined workflow for the Maxpar Direct Immune Profiling System.


Learn more about the Maxpar Direct Immune Profiling System

Introducing the new standard for human immune profiling
Michelle Poulin, PhD
Senior Manager, Field Applications

Immune cell profiling without compromise

The Maxpar Direct Immune Profiling Assay™ sets a new standard for comprehensive immunophenotyping of human PBMC and whole blood by enabling simultaneous identification and enumeration of 37 immune cell subsets (Figure 2).

A comprehensive immune profile

Figure 2. A comprehensive immune profile 
The 37 immune cell subsets identified using the Maxpar Direct Immune Profiling Assay and Maxpar Pathsetter software.

The highly multiplexed 30-marker antibody panel was developed with input from expert immunologists in academia and biopharma and builds on the panels designed by the Human ImmunoPhenotyping Consortium1.

Designed using industry-proven antibody clones, this assay provides you with the broadest single-tube view of the immune system from each precious sample.

You no longer need to compromise your study design due to the limitations of fluorescence cytometry.

Flexible by design

While the Maxpar Direct Immune Profiling Assay provides excellent immune cell coverage, you can easily add up to 14 new antibodies to support your study goals (Figure 3).

Because mass cytometry has limited channel crosstalk and a broad detection range, the expansion of high-parameter panels is simplified—a significant advantage over fluorescence cytometry.

Learn More about the Helios mass cytometer.

30 Markers

Figure 3. The 30 metal labels and associated antibody markers in the Maxpar Direct Immune Profiling Assay.
The 14 open channels are 106Cd, 110Cd, 111Cd, 112Cd, 113Cd, 114Cd, 116Cd, 142Nd, 159Tb, 162Dy, 165Ho, 169Tm, 175Lu and 209Bi. For a full list of antibodies, clones and metals in the panel, download the Technical Data Sheet.

Easily obtain labeled antibodies.

  • Choose from the expansive catalog of Maxpar metal-labeled antibodies.
  • Label your own antibodies using one of the Maxpar labeling kits.
  • Use the custom conjugation service for guaranteed antibody labeling performed by Fluidigm experts.


Maxpar Pathsetter: Your personal data analysis expert


Maxpar Pathsetter is a fully automated reporting and data analysis solution that automatically identifies 37 immune cell types in FCS files from samples processed with the Maxpar Direct Immune Profiling Assay.

Developed using probability state modeling, a proven high-parameter analytic approach2, the software eliminates the variability of manual gating and provides an efficient and reliable solution for longitudinal and multisite studies.

No more waiting for results—just click a button and go.


Contact us to learn more about the Maxpar Direct Immune Profiling System or request a seminar at your institution.
Provide us with your contact information below.


1. Maecker, H.T. et al. “Standardizing immunophenotyping for the Human Immunology Project.” Nature Reviews Immunology 12 (2012): 191–200.

2. Bagwell, C. Probability state modeling: a new paradigm for cytometric analysis. In: Litwin, V., Marder, P., editor. Flow Cytometry in Drug Discovery and Development. Hoboken NJ: John Wiley & Sons, Inc.; 2010. p 281.


Maxpar Direct Immune Profiling Poster 4.3MB PDF

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