Area of Interest Genomics Analysis PCR Applications Genotyping with Microfluidics

Genotyping 

Better DNA analysis begins with the best reagents 

Enhance your productivity with reagents designed to maximize the power of Juno™ and Biomark™  systems and reduce costs. The Juno workflow includes preamplification prior to genotyping, for an integrated process with minimal hands-on time. Use SNP Type™ or your existing TaqMan® assays with optimized reagents on all Fluidigm platforms. Juno™

Blue icon illustrating genotyping application

Methods 

SNP Type Genotyping 

SNP Type assays consist of unlabeled allele-specific primers and universal probes, making them an affordable alternative to probe-based genotyping assays. Lower price points make it possible to design and test for multiple markers and empirically choose the best. Select optimal assays designed for a variety of species using your supplied sequences. Optimized SNP Type preamplification protocols boost results from poor-quality, low-concentration or precious samples. 

TaqMan Genotyping

TaqMan probe-based SNP genotyping relies on dual-labeled probes and PCR primers. Each assay shares a pair of primers flanking the SNP and two or more allele-specific cleavage probes. Proximity suppresses fluorescent signaling until the probe specifically binds to the target. During PCR nuclease activity degrades the probe, resulting in increased brightness. Our reagents support TaqMan assays run on Biomark, EP1 and Juno platforms. 

Publications

Powering over 1,500 scientific publications with our products

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Dr. Dexi Chen, PhD, MD

Spotlight IMC DR. DEXI CHEN

Achieving multi-omic insights with microfluidics and CyTOF technology

Dr. Luc Teyton, MD, PhD

Spotlight MC DR. LUC TEYTON

How one lab explores the immune system one T cell at a time

Scott Magness, PhD

Spotlight MF SCOTT MAGNESS

Investigating transcriptomic changes at the microscale level

Simone Mayer, PhD

Spotlight MF Simone Mayer

Simone Mayer, PhD, explains how using multimodal analysis helped reveal new insights

Kelly Nudelman, PhD

Spotlight MF Kelly Nudelman and Tae-Hwi Schwantes

Kelly Nudelman and Tae-Hwi Schwantes-An discuss sample identification

Jo Vandesompele, PhD

Spotlight MF Jo Vandesompele

Jo Vandesompele at Ghent University discusses a deep dive into single-cell sequencing

Christophe Lancrin, PhD

Spotlight MF Christophe Lancrin

Christophe Lancrin on revealing new transcription factors using C1 and Biomark

Ansuman Satpathy, MD, PhD

Spotlight MF Ansu Satpathy

Stanford’s Ansu Satpathy designed single-cell T-ATAC-seq, now available at Script Hub

Shyam Prabhakar, PhD

Spotlight MC SHYAM PRABHAKAR

Shyam Prabhakar explores signatures of patient survival and EMT in colorectal tumors

customer stories

Learn more about how our customers are leveraging Fluidigm technology

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Unless explicitly and expressly stated otherwise, all Fluidigm products are provided for Research Use Only, not for use in diagnostic procedures. For more information see www.fluidigm.com/legal/notices