Advanta Immuno-Oncology Gene Expression Assay

Reveal the molecular signatures of tumor immune response

Feature

Highlights

Optimized
Screen high-value markers of the tumor immune response.

Flexible
Easily add new markers over time, customizing for your own research needs.

Efficient
Run up to 96 samples at a time using the proven Biomark HD automated qPCR system.

The Advanta™ Immuno-Oncology Gene Expression Assay is a 170-gene expression qPCR assay that enables profiling of tumor immunobiology and new biomarker identification.

Designed to meet the rigorous demands of human checkpoint research programs, the Advanta Immuno-Oncology Gene Expression Assay includes 91 key markers of tumor immune response that were shown in a multicenter international clinical trial to inform tumor progression and checkpoint therapeutic response. In collaboration with leading researchers and biopharma, this panel was further expanded to include 74 additional immuno-oncology markers.

The complete panel set includes genes for identification and functional analysis of immune and cancer cells including markers found in defined T cell subsets, cytokines and chemokines and markers of immune regulation, immune cell fate and more.

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Immuno-Oncology Expression Assay

Panel A

ARG1 CLEC4C IL12A PDCD1LG2 (PD-L2)
BTLA CSF2 IL13 PRF1
CCL2 CTLA4 IL17A PTGER2
CCL22 CX3CL1 IL17F PTGER4
CCL28 CXCL10 IL1B PTGS2
CCR5 CXCL8 IL2 PTPRC
CCR7 CXCL9 IL2RA RORC
CD1C CXCR3 IL4 SDHA
CD244 EOMES IL6 SP2
CD27 EPCAM IL7 TBX21
CD274 (PD-L1) FOXP3 IL7R TGFB1
CD276 GZMA ITGAM TMEM55B
CD28 GZMB ITGAX TNF
CD3E HAVCR2 ITGB2 TNFRSF14
CD4 HLA-A KLRK1 TNFRSF4
CD40 HLA-B LAG3 TNFRSF9
CD40LG HLA-C LGALS9 TNFSF4
CD48 HMOX1 MAP4K1 TNFSF9
CD69 ICAM1 MICA VCAM1
CD70 ICOS MICB VEGFA
CD80 IDO1 MS4A1 VPS33B
CD86 IFNG NCAM1 VTCN1
CD8A IL10 PDCD1 (PD-1)

Panel B

APOBEC3A CXCR4 IFNA2 NKG7
APOBEC3B CYBB IGHA1 NRAS
ARG2 DGAT2 IGHG1 NT5E
CA4 EBI3 IGHM PYGL
CCL18 ERBB2 JCHAIN (IGJ) SLAMF7
CCL21 FASLG IGKC SLAMF8
CCL3 FCER1G IGLJ3 STAT1
CCL4 FCRLA IGSF6 STAT2
CCL5 FYB IL10RA STAT3
CD160 GATA3 IL12B STAT5A
CD19 GNLY IL15 STAT5B
CD1D GZMH IL2RG STAT6
CD2 GZMK IRF9 TLR7
CD22 HLA-DMB ISG15 TLR8
CD37 HLA-DPB1 JAK2 TNFAIP8
CD52 HLA-DQB1 KREMEN1 TNFRSF18
CD53 HLA-DRB1 LAPTM5 TNFSF18
CD63 IFI27 LCK
CTSS IFIT2 LRG1

Housekeeping genes for panels A and B:
B2M, ACTB, GAPDH, GUSB, TFRC

Table 1. The Advanta Immuno-Oncology Gene Expression Assay panels A and B. This two panel set includes 91 target genes and 5 housekeeping genes on Panel A and 74 target genes and the same 5 housekeeping genes on Panel B. Panel B reserves 17 open assay inlets, so you have the added flexibility to add new assays over time for customization. Each panel will be available in packs of 2 or 10 (192 or 960 samples, respectively.

The Biomark HD advantage

The Advanta Immuno-Oncology Gene Expression Assay is optimized to run on the industry-proven Biomark HD system. This system utilizes integrated fluidic circuits (IFCs) to precisely combine multiple reactions at nanoliter volumes. Enabling you to easily run up to 96 samples at a time with the Advanta Immuno-Oncology Gene Expression Assay, the Biomark HD automated gene expression workflow delivers performance you can trust with limited hands-on time.

BMK M 96.96 GT IFC

Figure 1. Integrated fluidic circuit (IFC). The Gene Expression 96.96 IFC accommodates up to 96 samples per run, with a total of 9,216 parallel reactions. This is the equivalent reaction number of 24 total 384-well plates.

Assay workflow

Advanta IO Assay Workflow

Figure 2. Assay workflow. The gene expression assay workflow uses the Juno™ system for IFC preparation and Biomark HD for qPCR thermal cycling and data acquisition. With only 30 minutes of hands-on time and four hours of total workflow time, you can run up to 96 samples using the GE 96.96 IFC, generating results for up to 9,216 PCR reactions.

Advanta Immuno-Oncology Gene Expression Assay ordering info

Part Number

Part Description

Content

101-6082 Advanta Immuno-Oncology Gene Expression Assay Panel A—2 IFCs Reagents, assay, syringes, IFCs
101-6083 Advanta Immuno-Oncology Gene Expression Assay Panel B—2 IFCs Reagents, assay, syringes, IFCs
101-6084 Advanta Immuno-Oncology Gene Expression Assay Panels A & B—4 IFCs Reagents, assay, syringes, IFCs

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For Research Use Only. Not for use in diagnostic procedures.